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Recombinant fragment, corresponding to a region within amino acids 240-457 of Human ALDH1A2.
Our Abpromise guarantee covers the use of ab96060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
ALDH1A2 was immunoprecipitated using 0.5mg Mouse Kidney tissue extract, 5µg of Rabbit polyclonal to ALDH1A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Kidney tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96060.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 57kDa; ALDH1A2
This image is courtesy of an anonymous AbreviewBlocked with 5% Milk,Primary Incubation 3 hours at room temperature, diluent 5% milk in TBS-Tween-20 (0.05%).
This image is courtesy of an anonymous AbreviewBlocked with 5% milk
ab96060 has not yet been referenced specifically in any publications.