Recombinant
RabMAb

Anti-ALDH1A2 antibody [EPR9370] (ab156019)

Overview

  • Product name
    Anti-ALDH1A2 antibody [EPR9370]
    See all ALDH1A2 primary antibodies
  • Description
    Rabbit monoclonal [EPR9370] to ALDH1A2
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human ALDH1A2 (UniProt O94788).

  • Positive control
    • Rat testis, Mouse testis, Molt4 and K562 lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab156019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently.
    • Pathway
      Cofactor metabolism; retinol metabolism.
    • Sequence similarities
      Belongs to the aldehyde dehydrogenase family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AL1A2_HUMAN antibody
      • Aldehyde dehydrogenase family 1 member A2 antibody
      • ALDH1A2 aldehyde dehydrogenase 1 family, member A2 antibody
      • ALDH1A2 antibody
      • Aldh1a7 antibody
      • AV116159 antibody
      • MGC26444 antibody
      • RALDH 2 antibody
      • RALDH(II) antibody
      • Raldh1 antibody
      • RalDH2 antibody
      • RALDH2 T antibody
      • Retinal dehydrogenase 2 antibody
      • Retinaldehyde dehydrogenase 2 antibody
      • Retinaldehyde specific dehydrogenase type 2 antibody
      • Retinaldehyde-specific dehydrogenase type 2 antibody
      see all

    Images

    • All lanes : Anti-ALDH1A2 antibody [EPR9370] (ab156019) at 1/1000 dilution

      Lane 1 : Rat testis lysate
      Lane 2 : Mouse testis lysate
      Lane 3 : Molt4 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 57 kDa

    References

    This product has been referenced in:
    • Yasgar A  et al. A High-Content Assay Enables the Automated Screening and Identification of Small Molecules with Specific ALDH1A1-Inhibitory Activity. PLoS One 12:e0170937 (2017). Read more (PubMed: 28129349) »
    • Lee H  et al. Fusion protein of retinol-binding protein and albumin domain III reduces liver fibrosis. EMBO Mol Med 7:819-30 (2015). WB . Read more (PubMed: 25864124) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10%)
    Loading amount
    15 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 19 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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