Anti-ALDH1L1 antibody - Astrocyte Marker (ab87117)

Overview

  • Product nameAnti-ALDH1L1 antibody - Astrocyte Marker
    See all ALDH1L1 primary antibodies
  • Description
    Rabbit polyclonal to ALDH1L1 - Astrocyte Marker
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Mouse ALDH1L1.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse liver; Mouse brain; Rat brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab87117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 99 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
IHC-Fr 1/1000.
ICC Use at an assay dependent concentration.

Target

  • Tissue specificityHighly expressed in liver, pancreas and kidney.
  • Sequence similaritiesIn the N-terminal section; belongs to the GART family.
    In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
    Contains 1 acyl carrier domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 10 formyltetrahydrofolate dehydrogenase antibody
    • 10 FTHFDH antibody
    • 10-formyltetrahydrofolate dehydrogenase antibody
    • 10-FTHFDH antibody
    • AL1L1_HUMAN antibody
    • Aldehyde dehydrogenase 1 family member L1 antibody
    • Aldehyde dehydrogenase family 1 member L1 antibody
    • aldh1l1 antibody
    • Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
    • DKFZp781N0997 antibody
    • EC 1.5.1.6 antibody
    • FDH antibody
    • formyltetrahydrofolate dehydrogenase antibody
    • FTHFD antibody
    • OTTHUMP00000155221 antibody
    • OTTHUMP00000214696 antibody
    • OTTHUMP00000214700 antibody
    • OTTHUMP00000222724 antibody
    see all

Anti-ALDH1L1 antibody - Astrocyte Marker images

  • All lanes : Anti-ALDH1L1 antibody - Astrocyte Marker (ab87117) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes
  • IHC-P image of ALDH1L1 staining on Mouse brain tissue section using ab87117. The sections were subjected to heat-mediated antigen retrieval using citric acid (pH 6). The sections were blocked using 1% BSA for 10 minutes at 21°C and then incubated with ab87117 (1/1000 dilution) for 2 hours at 21°C.

    See Abreview

  • ab87117 staining ALDH1L1 in mouse brain-astrocytes tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, incubated with 30% sucrose, embedded in OCT and blocked with 5% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 0.3% Triton + 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-ALDH1L1 antibody - Astrocyte Marker (ab87117)

This product has been referenced in:
  • Shigemoto-Mogami Y  et al. Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone. J Neurosci 34:2231-43 (2014). WB, ICC/IF ; Rat . Read more (PubMed: 24501362) »
  • Tong X  et al. Astrocyte Kir4.1 ion channel deficits contribute to neuronal dysfunction in Huntington's disease model mice. Nat Neurosci 17:694-703 (2014). Read more (PubMed: 24686787) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Sample Mouse Tissue sections (brain-astrocytes)
Specification brain-astrocytes
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 05 2014

I am very pleased to hear you would like to accept our offer and test ab87117 in human samples.

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for human samples an...

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Thank you for your reply.

ab87117 is suitable for paraffin-embedded sections but it hasnt been tested on human samples yet. Therefore, I can offer a discount off a future purchase if you buy ab87117 now, test it in human and submit feedback ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (mouse cortex)
Specification mouse cortex
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% triton x100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 29 2012

Thank you for providing that information. I have now generated the discount code for your customer:
Discount code: xxxxxx
Expiration date: 11th July 2012
In order to activate the code, please advise your customer to submit an Abreview for...

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Thank you for contacting us.

Yes, that information would be very valuable for us and our customers. Ab87117 has previously been used by one of our customers to perform ICC with rat cultured cells. Although the results were not as hoped, the ...

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Application Immunocytochemistry
Sample Rat Cultured Cells (Astrocytes)
Specification Astrocytes
Fixative Formaldehyde
Permeabilization Yes - Triton-X100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 50% · Temperature: 24°C
Username

Rehana Leak

Verified customer

Submitted Jul 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain cortex)
Specification Brain cortex
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted May 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Feb 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"