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Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Mouse ALDH1L1.
Our Abpromise guarantee covers the use of ab87117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 99 kDa).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 10 µg/ml. PubMed: 24501362|
|ICC||Use at an assay dependent concentration.|
ab87117 staining ALDH1L1 in mouse brain-astrocytes tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, incubated with 30% sucrose, embedded in OCT and blocked with 5% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 0.3% Triton + 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Paraffin-embedded rat brain tissue stained for ALDH1L1 using ab87117 at 1/1000 dilution in immunohistochemical analysis.
Astrocytes are clearly labeled: red for those attaching to the pia mater, blue for those in the grey matter and green for astrocytes that are intimately associated with blood vessels.