Recombinant
RabMAb

Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)

Overview

  • Product name
    Anti-ALDH1L1 antibody [EPR12743(B)]
    See all ALDH1L1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12743(B)] to ALDH1L1
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ALDH1L1 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75891

  • Positive control
    • HepG2, 293T, fetal brain, fetal kidney and fetal liver lysates. Human kidney and Human liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab177463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Tissue specificity
      Highly expressed in liver, pancreas and kidney.
    • Sequence similarities
      In the N-terminal section; belongs to the GART family.
      In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
      Contains 1 acyl carrier domain.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 10 formyltetrahydrofolate dehydrogenase antibody
      • 10 FTHFDH antibody
      • 10-formyltetrahydrofolate dehydrogenase antibody
      • 10-FTHFDH antibody
      • AL1L1_HUMAN antibody
      • Aldehyde dehydrogenase 1 family member L1 antibody
      • Aldehyde dehydrogenase family 1 member L1 antibody
      • aldh1l1 antibody
      • Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
      • DKFZp781N0997 antibody
      • EC 1.5.1.6 antibody
      • FDH antibody
      • formyltetrahydrofolate dehydrogenase antibody
      • FTHFD antibody
      • OTTHUMP00000155221 antibody
      • OTTHUMP00000214696 antibody
      • OTTHUMP00000214700 antibody
      • OTTHUMP00000222724 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab177463 at 1/50 dilution. 

    • All lanes : Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Fetal brain lysate
      Lane 4 : Fetal kidney lysate
      Lane 5 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 99 kDa
    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH1L1 with ab177463 at 1/50 dilution.

    • All lanes : Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463) at 1/1000 dilution

      Lane 1 : Fetal liver lysate at 10 µg
      Lane 2 : 1X PBS (negative control)

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 99 kDa

    References

    This product has been referenced in:
    • Raddatz BB  et al. Transcriptional analysis of glial cell differentiation in the postnatal murine spinal cord. Int J Dev Neurosci 42:24-36 (2015). IHC-P ; Mouse . Read more (PubMed: 25702526) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (CNS - Medulla)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    CNS - Medulla
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Jim Manavis

    Verified customer

    Submitted Aug 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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