Overview

  • Product nameAnti-ALDH2 antibody [9A10AF10]
    See all ALDH2 primary antibodies
  • Description
    Mouse monoclonal [9A10AF10] to ALDH2
  • Tested applicationsSuitable for: ICC/IF, IP, In-Cell ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Tissue, cells or virus corresponding to ALDH2. Rat liver mitochondria.

  • Positive control
    • HepG2 cells; Human, Rat and Mouse liver and HepG2 cell lysates.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml. For 2 hours.
IP Use at an assay dependent dilution.
In-Cell ELISA Use a concentration of 5 µg/ml.

Target

Anti-ALDH2 antibody [9A10AF10] images

  • Immunocytochemistry analysis using ab110311 at 5µg/ml staining ALDH2 in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
    Note: Target protein locates mainly in the mitochondrial matrix.
  • Using MitoSciences' standard immunoprecipitation protocol, ab110311 precipitates down the 54kDa mature ALDH2 protein from human, mouse, and rat liver tissue lysate and HepG2 culture cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

References for Anti-ALDH2 antibody [9A10AF10] (ab110311)

ab110311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"