Overview

  • Product nameAnti-ALDH2 antibody
    See all ALDH2 primary antibodies
  • Description
    Rabbit polyclonal to ALDH2
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N-terminal region of human ALDH2.

  • Positive control
    • Paraffin embedded Human hepatocarcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab70917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21280157
WB 1/50 - 1/100. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • PathwayAlcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
  • Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetaldehyde dehydrogenase 2 antibody
    • Aldehyde dehydrogenase 2 family (mitochondrial) antibody
    • Aldehyde dehydrogenase 2 family antibody
    • Aldehyde dehydrogenase mitochondrial antibody
    • Aldehyde dehydrogenase, mitochondrial antibody
    • ALDH 2 antibody
    • ALDH class 2 antibody
    • ALDH E2 antibody
    • ALDH-E2 antibody
    • Aldh2 antibody
    • ALDH2_HUMAN antibody
    • ALDHI antibody
    • ALDM antibody
    • Liver mitochondrial ALDH antibody
    • MGC1806 antibody
    • Mitochondrial aldehyde dehydrogenase 2 antibody
    • MS767 antibody
    • Nucleus encoded mitochondrial aldehyde dehydrogenase 2 antibody
    see all

Anti-ALDH2 antibody images

  • All lanes : Anti-ALDH2 antibody (ab70917) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell line lysates
    Lane 2 : 293 cell line lysates transiently transfected with the ALDH2 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab70917, at a 1/10 dilution, staining human ALDH2 in hepatocarcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70917 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
  • All lanes : Anti-ALDH2 antibody (ab70917) at 1/100 dilution

    Lane 1 : Natural human Alpha 2 Antiplasmin protein (ab77936) at 0.1 µg
    Lane 2 : Natural human Alpha 2 Antiplasmin protein (ab77936) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Anti-ALDH2 antibody (ab70917)

This product has been referenced in:
  • Marcato P  et al. Aldehyde Dehydrogenase Activity of Breast Cancer Stem Cells Is Primarily Due To Isoform ALDH1A3 and Its Expression Is Predictive of Metastasis. Stem Cells 29:32-45 (2011). WB, ICC/IF, IHC-P ; Human . Read more (PubMed: 21280157) »

See 1 Publication for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (cultured choroid plexus cells)
Loading amount 15 µg
Specification cultured choroid plexus cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Human cerebral cortex and choroid plexus lysate)
Loading amount 20 µg
Specification Human cerebral cortex and choroid plexus lysate
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2013

Thank you for your inquiry.

I agree that this antibody could cross-react with human retinal dehydrogenase(ALDH1A1) too, since there is a homology of 76%.

I believe this could be of biological relevance. The different bands could be ...

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Thank you very much for your valuable feedback.

Indeed, as the immunogen sequence and the rat protein show a 100% similarity, it is very likely that the antibody will cross-react. However, please be aware thatthe similarity with the retinal d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"