Human, Common marmoset Predicted to work with:
Recombinant fragment, corresponding to amino acids 166-400 of Human ALDH3A2 (BC002430).
293T cell lysate, A549 cell lysate, Human fetal testis lysate, Human fetal skeletal muscle tissue.
Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
1/100 - 1/500.
Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This gene product catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid.
Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS). SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects.
ab113111, at 1/100 dilution, staining ALDH3A2 in formalin-fixed, paraffin-embedded Human Fetal skeletal muscle by Immunohistochemistry.
Western blot - ALDH3A2 antibody (ab113111)
All lanes : Anti-ALDH3A2 antibody (ab113111) at 1/1000 dilution
Lane 1 : 293T cell lysate Lane 2 : A549 cell lysate Lane 3 : Human fetal testis lysate
Predicted band size : 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A2 antibody (ab113111)This image is courtesy of an Abreview from Zachary Yu-Ching Lin.
IHC-P image of ALDH3A2 staining with ab113111 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab113111 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
Xiao N et al. A novel aldehyde dehydrogenase-3 activator (Alda-89) protects submandibular gland function from irradiation without accelerating tumor growth. Clin Cancer Res19:4455-64 (2013).
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