Overview

  • Product name
  • Description
    Rabbit polyclonal to ALDH3A2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Common marmoset
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 166-400 of Human ALDH3A2 (BC002430).

  • Positive control
    • 293T cell lysate, A549 cell lysate, Human fetal testis lysate, Human fetal skeletal muscle tissue.

Properties

  • Form
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 99% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab113111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/100 - 1/500.

Target

  • Relevance
    Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This gene product catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS). SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects.
  • Cellular localization
    Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side.
  • Database links
  • Alternative names
    • Aldehyde dehydrogenase antibody
    • Aldehyde dehydrogenase 10 antibody
    • Aldehyde dehydrogenase 3 family member A2 antibody
    • ALDH10 antibody
    • ALDH3A2 antibody
    • DKFZp686E23276 antibody
    • FALDH antibody
    • Fatty aldehyde dehydrogenase antibody
    • FLJ20851 antibody
    • Microsomal aldehyde dehydrogenase antibody
    • OTTHUMP00000065799 antibody
    • OTTHUMP00000065800 antibody
    • OTTHUMP00000065801 antibody
    • SLS antibody
    see all

Images

  • ab113111, at 1/100 dilution, staining ALDH3A2 in formalin-fixed, paraffin-embedded Human Fetal skeletal muscle by Immunohistochemistry.
  • All lanes : Anti-ALDH3A2 antibody (ab113111) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : Human fetal testis lysate


    Predicted band size : 55 kDa
  • IHC-P image of ALDH3A2 staining with ab113111 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab113111 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:
  • Kang JH  et al. Aldehyde dehydrogenase is used by cancer cells for energy metabolism. Exp Mol Med 48:e272 (2016). WB ; Human . Read more (PubMed: 27885254) »
  • Xiao N  et al. A novel aldehyde dehydrogenase-3 activator (Alda-89) protects submandibular gland function from irradiation without accelerating tumor growth. Clin Cancer Res 19:4455-64 (2013). Read more (PubMed: 23812668) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (Adult testis)
Specification
Adult testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Apr 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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