Recombinant
RabMAb

Anti-ALDH4A1 antibody [EPR14287] (ab185208)

Overview

  • Product name
    Anti-ALDH4A1 antibody [EPR14287]
    See all ALDH4A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14287] to ALDH4A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ALDH4A1 aa 50-150. The exact sequence is proprietary.
    Database link: P30038

  • Positive control
    • Human fetal hear and HepG2 lysate; Human liver and Mouse skeletal muscle tissue;. HeLa and HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/100 - 1/250.
Flow Cyt 1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250.

Target

  • Function
    Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.
  • Tissue specificity
    Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.
  • Pathway
    Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
  • Involvement in disease
    Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2) [MIM:239510]. HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL4A1_HUMAN antibody
    • aldehyde dehydrogenase 4 antibody
    • aldehyde dehydrogenase 4 family, member A1 antibody
    • Aldehyde dehydrogenase family 4 member A1 antibody
    • Aldehyde dehydrogenase, family 4, subfamily A, member 1 antibody
    • ALDH4 antibody
    • aldh4a1 antibody
    • Delta 1 pyrroline 5 carboxylate dehydrogenase, mitochondrial antibody
    • Delta-1-pyrroline-5-carboxylate dehydrogenase antibody
    • L-glutamate gamma-semialdehyde dehydrogenase antibody
    • mitochondrial antibody
    • mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase antibody
    • P5C dehydrogenase antibody
    • P5CD antibody
    • P5CDh antibody
    • P5CDhL antibody
    • P5CDhS antibody
    • Pyrroline-5-carboxylate dehydrogenase antibody
    • RP11 128M10.1 antibody
    see all

Images

  • Anti-ALDH4A1 antibody [EPR14287] (ab185208) at 1/1000 dilution + Human fetal heart at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH4A1 with ab185208 at 1/250 dilution. Counter stain Hematoxylin.

  • Immunofluorescent analysis of -20℃ acetone fixed HeLa cells labeling ALDH4A1 with ab185208 at 1/250 dilution (red). Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi (blue).

  • Flow cytometric analysis of 2% paraformaldehyde fixed HepG2 cells labeling ALDH4A1 with ab185208 at 1/160 dilution (red), or a rabbit IgG isotype control (green), followed by secondary goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Anti-ALDH4A1 antibody [EPR14287] (ab185208) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling ALDH4A1 with ab185208 at 1/250 dilution. Counter stain Hematoxylin.

References

ab185208 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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