Overview

  • Product name
  • Description
    Rabbit polyclonal to ALDH5A1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 85-324 of Human ALDH5A1 (Uniprot ID: P51649).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji, Mouse brain whole cell lysate; A431 cells; A549 xenograft
  • General notes
    Keep as a concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).
  • Tissue specificity
    Brain, pancreas, heart, liver, skeletal muscle and kidney. Lower in placenta.
  • Pathway
    Amino-acid degradation; 4-aminobutanoate degradation.
  • Involvement in disease
    Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency) [MIM:271980]. SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aldedehyde dehydrogenase 5 family antibody
    • Aldehyde dehydrogenase 5 family member A1 antibody
    • Aldehyde dehydrogenase 5A1 antibody
    • Aldehyde dehydrogenase family 5 member A1 antibody
    • ALDH5A 1 antibody
    • Aldh5a1 antibody
    • mitochondrial antibody
    • Mitochondrial succinate semialdehyde dehydrogenase antibody
    • NAD(+) dependent succinic semialdehyde dehydrogenase antibody
    • NAD(+)-dependent succinic semialdehyde dehydrogenase antibody
    • SSADH antibody
    • SSDH antibody
    • SSDH_HUMAN antibody
    • Succinate semialdehyde dehydrogenase antibody
    • Succinate-semialdehyde dehydrogenase antibody
    see all

Images

  • Anti-ALDH5A1 antibody (ab155493) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 57 kDa
  • Anti-ALDH5A1 antibody (ab155493) at 1/5000 dilution + Mouse brain whole cell lysate at 20 µg

    Predicted band size : 57 kDa
  • Immunofluorescent analysis of methanol-fixed A431 cells labeling ALDH5A1 with ab155493 at 1/500 dilution. Lower panel co-stained with Hoescht 33342.
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling ALDH5A1 with ab155493 at 1/500 dilution.

References

ab155493 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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