Overview

  • Product name
  • Description
    Rabbit polyclonal to ALDH5A1
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N-terminal region of human ALDH5A1.

Properties

Applications

Our Abpromise guarantee covers the use of ab65469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21280157
WB 1/50 - 1/100. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/10 - 1/50.
ELISA 1/1000.

Target

  • Function
    Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).
  • Tissue specificity
    Brain, pancreas, heart, liver, skeletal muscle and kidney. Lower in placenta.
  • Pathway
    Amino-acid degradation; 4-aminobutanoate degradation.
  • Involvement in disease
    Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency) [MIM:271980]. SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aldedehyde dehydrogenase 5 family antibody
    • Aldehyde dehydrogenase 5 family member A1 antibody
    • Aldehyde dehydrogenase 5A1 antibody
    • Aldehyde dehydrogenase family 5 member A1 antibody
    • ALDH5A 1 antibody
    • Aldh5a1 antibody
    • mitochondrial antibody
    • Mitochondrial succinate semialdehyde dehydrogenase antibody
    • NAD(+) dependent succinic semialdehyde dehydrogenase antibody
    • NAD(+)-dependent succinic semialdehyde dehydrogenase antibody
    • SSADH antibody
    • SSDH antibody
    • SSDH_HUMAN antibody
    • Succinate semialdehyde dehydrogenase antibody
    • Succinate-semialdehyde dehydrogenase antibody
    see all

Images

  • All lanes : Anti-ALDH5A1 antibody (ab65469) at 1/50 dilution

    Lane 1 : 293 cell lysates
    Lane 2 : 293 cell lysates, transfected with the ALDH5A1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Ab65469 (1:10) staining human ALDH5A1 in human hepatocarcinoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

References

This product has been referenced in:
  • Marcato P  et al. Aldehyde Dehydrogenase Activity of Breast Cancer Stem Cells Is Primarily Due To Isoform ALDH1A3 and Its Expression Is Predictive of Metastasis. Stem Cells 29:32-45 (2011). ICC/IF ; Human . Read more (PubMed: 21280157) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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