The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 58 kDa.
FunctionPlays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
Involvement in diseaseDefects in ALDH6A1 are the cause of methylmalonate semialdehyde dehydrogenase deficiency (MMSDH deficiency) [MIM:603178]. This is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
ab109582 (5µg/ml) shows staining of ALDH6A1 in paraffin embedded Human Liver following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows textured staining of the cytoplasm consistent with mitochondria.
Western blot - ALDH6A1 antibody (ab109582)
Anti-ALDH6A1 antibody (ab109582) at 0.1 µg/ml + Mouse Kidney lysate (in RIPA buffer) at 35 µg
Predicted band size : 58 kDa
References for Anti-ALDH6A1 antibody (ab109582)
has not yet been referenced specifically in any publications.
Publishing research using ab109582? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"