Overview

  • Product nameAnti-ALDH6A1 antibody
    See all ALDH6A1 primary antibodies
  • Description
    Rabbit polyclonal to ALDH6A1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C-terminal region of human ALDH6A1.

Properties

Applications

Our Abpromise guarantee covers the use of ab65471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21280157
WB 1/50 - 1/100. Detects a band of approximately 56 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/10 - 1/50.
ELISA 1/1000.

Target

  • FunctionPlays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
  • Involvement in diseaseDefects in ALDH6A1 are the cause of methylmalonate semialdehyde dehydrogenase deficiency (MMSDH deficiency) [MIM:603178]. This is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
  • Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110038I05Rik antibody
    • AI314632 antibody
    • Aldehyde dehydrogenase 6 family member A1 antibody
    • Aldehyde dehydrogenase family 6 member A1 antibody
    • Aldehyde dehydrogenase family 6, subfamily A1 antibody
    • ALDH6A1 antibody
    • Malonate semialdehyde dehydrogenase antibody
    • Malonate-semialdehyde dehydrogenase [acylating] antibody
    • Methylmalonate semialdehyde dehydrogenase [acylating] mitochondrial antibody
    • Methylmalonate-semialdehyde dehydrogenase [acylating] antibody
    • MGC37325 antibody
    • MGC40271 antibody
    • Mitochondrial acylating methylmalonate semialdehyde dehydrogenase antibody
    • mitochondrial antibody
    • MMSA_HUMAN antibody
    • MMSADHA antibody
    • MMSDH antibody
    see all

Anti-ALDH6A1 antibody images

  • All lanes : Anti-ALDH6A1 antibody (ab65471) at 1/50 dilution

    Lane 1 : 293 cell lysate, nontransfected
    Lane 2 : 293 cell lysate, transiently transfected with the ALDH6A1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 58 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • Ab65471 (1:10) staining human ALDH6A1 in human hepatocarcinoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

References for Anti-ALDH6A1 antibody (ab65471)

This product has been referenced in:
  • Marcato P  et al. Aldehyde Dehydrogenase Activity of Breast Cancer Stem Cells Is Primarily Due To Isoform ALDH1A3 and Its Expression Is Predictive of Metastasis. Stem Cells 29:32-45 (2011). ICC/IF ; Human . Read more (PubMed: 21280157) »

See 1 Publication for this product

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