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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ALDH7A1 aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: P49419
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Our Abpromise guarantee covers the use of ab53278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/50000 - 1/100000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||Use at an assay dependent concentration.|
Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) labelling ALDH7A1 with purified ab53278 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
ab53278 has not yet been referenced specifically in any publications.