Overview

  • Product nameAnti-ALDH8A1 antibody
  • Description
    Mouse polyclonal to ALDH8A1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human) ALDH8A1 (NP_072090.1).

  • Positive control
    • ALDH8A1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • FunctionConverts 9-cis-retinal to 9-cis-retinoic acid. Has lower activity towards 13-cis-retinal. Has much lower activity towards all-trans-retinal. Has highest activity with benzaldehyde and decanal (in vitro). Has a preference for NAD, but shows considerable activity with NADP (in vitro).
  • Tissue specificityHighly expressed in adult kidney and liver. Detected at lower levels in fetal liver and kidney.
  • Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL8A1_HUMAN antibody
    • Aldehyde dehydrogenase 12 antibody
    • Aldehyde dehydrogenase 8 family, member A1 antibody
    • Aldehyde dehydrogenase family 8 member A1 antibody
    • ALDH12 antibody
    • Aldh8a1 antibody
    • DJ352A20.2 antibody
    • DKFZp779D2315 antibody
    • MGC138650 antibody
    • Raldh4 antibody
    see all

Anti-ALDH8A1 antibody images

  • All lanes : Anti-ALDH8A1 antibody (ab68773) at 1/500 dilution

    Lane 1 : ALDH8A1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

References for Anti-ALDH8A1 antibody (ab68773)

ab68773 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Loading amount 50 µg
Specification HEK293T
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"