The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 54 kDa.
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionConverts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine. Catalyzes the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction.
Tissue specificityHigh expression in adult liver, skeletal muscle, and kidney. Low levels in heart, pancreas, lung and brain. Expressed in all regions of the brain. Expression levels are variable in the different brain areas, with the highest levels in the spinal cord and the lowest in the occipital pole.
PathwayAmine and polyamine biosynthesis; carnitine biosynthesis.
Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
Developmental stageStrongly expressed in human embryonic brain (gestational age 12 weeks).
Post-translational modificationsThe N-terminus is blocked.
ab103898 (3.8µg/ml) staining of paraffin embedded Human Cerebellum following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows staining of a few cells between the Granular Cell layer and the Molecular Layer of the Cerebellum, note the unstained Purkinje cell at 10 O'clock.
Western blot - ALDH9A1 antibody (ab103898)
Anti-ALDH9A1 antibody (ab103898) at 0.3 µg/ml + Human kidney lysate at 35 µg