Overview

  • Product nameAnti-ALDH9A1 antibody
    See all ALDH9A1 primary antibodies
  • Description
    Rabbit polyclonal to ALDH9A1
  • Tested applicationsSuitable for: WB, Indirect ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human)from the C-terminal region of human ALDH9A1 conjugated to KLH.

  • Positive control
    • Mouse kidney tissue lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab79726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 54 kDa.
Indirect ELISA 1/1000.
ELISA Use at an assay dependent concentration.

Target

  • FunctionConverts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine. Catalyzes the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction.
  • Tissue specificityHigh expression in adult liver, skeletal muscle, and kidney. Low levels in heart, pancreas, lung and brain. Expressed in all regions of the brain. Expression levels are variable in the different brain areas, with the highest levels in the spinal cord and the lowest in the occipital pole.
  • PathwayAmine and polyamine biosynthesis; carnitine biosynthesis.
  • Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
  • Developmental stageStrongly expressed in human embryonic brain (gestational age 12 weeks).
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4 trimethylaminobutyraldehyde dehydrogenase antibody
    • 4-trimethylaminobutyraldehyde dehydrogenase antibody
    • AL9A1_HUMAN antibody
    • aldehyde dehydrogenase (NAD+) antibody
    • Aldehyde dehydrogenase 9A1 antibody
    • Aldehyde dehydrogenase E3 isozyme antibody
    • Aldehyde dehydrogenase family 9 member A1 antibody
    • ALDH4 antibody
    • ALDH7 antibody
    • ALDH9 antibody
    • aldh9A1 antibody
    • E3 antibody
    • Gamma aminobutyraldehyde dehydrogenase antibody
    • Gamma-aminobutyraldehyde dehydrogenase antibody
    • R aminobutyraldehyde dehydrogenase antibody
    • R-aminobutyraldehyde dehydrogenase antibody
    • TMABADH antibody
    see all

Anti-ALDH9A1 antibody images

  • Anti-ALDH9A1 antibody (ab79726) at 1/50 dilution + mouse kidney tissue lysate at 35 µg

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

References for Anti-ALDH9A1 antibody (ab79726)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Sample Human Tissue lysate - whole (brain)
Total protein in input 500 µg
Specification brain
Immuno-precipitation step Other - dynabead
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Submitted May 04 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (brain)
Specification brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton x-100
Blocking step FBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted May 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (BRAIN)
Loading amount 50 µg
Specification BRAIN
Gel Running Conditions Non-reduced Denaturing (12%PA GEL)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
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Submitted Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Serum (blood)
Specification blood
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C
Type Direct
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Submitted Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (neuronal cell line HpL3-4)
Loading amount 30 µg
Specification neuronal cell line HpL3-4
Gel Running Conditions Non-reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
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Submitted Oct 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"