Overview

  • Product name
  • Description
    Rabbit polyclonal to ALDH9A1
  • Tested applications
    Suitable for: WB, Indirect ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human)from the C-terminal region of human ALDH9A1 conjugated to KLH.

  • Positive control
    • Mouse kidney tissue lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab79726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 54 kDa.
Indirect ELISA 1/1000.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine. Catalyzes the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction.
  • Tissue specificity
    High expression in adult liver, skeletal muscle, and kidney. Low levels in heart, pancreas, lung and brain. Expressed in all regions of the brain. Expression levels are variable in the different brain areas, with the highest levels in the spinal cord and the lowest in the occipital pole.
  • Pathway
    Amine and polyamine biosynthesis; carnitine biosynthesis.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Developmental stage
    Strongly expressed in human embryonic brain (gestational age 12 weeks).
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4 trimethylaminobutyraldehyde dehydrogenase antibody
    • 4-trimethylaminobutyraldehyde dehydrogenase antibody
    • AL9A1_HUMAN antibody
    • aldehyde dehydrogenase (NAD+) antibody
    • Aldehyde dehydrogenase 9A1 antibody
    • Aldehyde dehydrogenase E3 isozyme antibody
    • Aldehyde dehydrogenase family 9 member A1 antibody
    • ALDH4 antibody
    • ALDH7 antibody
    • ALDH9 antibody
    • aldh9A1 antibody
    • E3 antibody
    • Gamma aminobutyraldehyde dehydrogenase antibody
    • Gamma-aminobutyraldehyde dehydrogenase antibody
    • R aminobutyraldehyde dehydrogenase antibody
    • R-aminobutyraldehyde dehydrogenase antibody
    • TMABADH antibody
    see all

Anti-ALDH9A1 antibody images

  • Anti-ALDH9A1 antibody (ab79726) at 1/50 dilution + mouse kidney tissue lysate at 35 µg

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

References for Anti-ALDH9A1 antibody (ab79726)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application
Immunoprecipitation
Sample
Human Tissue lysate - whole (brain)
Total protein in input
500 µg
Specification
brain
Immuno-precipitation step
Other - dynabead
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Abcam user community

Verified customer

Submitted May 04 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (brain)
Specification
brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton x-100
Blocking step
FBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted May 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (BRAIN)
Loading amount
50 µg
Specification
BRAIN
Gel Running Conditions
Non-reduced Denaturing (12%PA GEL)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Serum (blood)
Specification
blood
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C
Type
Direct
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Abcam user community

Verified customer

Submitted Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal cell line HpL3-4)
Loading amount
30 µg
Specification
neuronal cell line HpL3-4
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Oct 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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