Anti-ALDOB antibody [5E2AD2] (ab129728)

Overview

• Product nameAnti-ALDOB antibody [5E2AD2]See all ALDOB primary antibodies ...
• Description
  Mouse monoclonal [5E2AD2] to ALDOB
• Tested applicationsIP, ICC/IF, In-Cell ELISA, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Purified Human liver mitochondria

• Positive controlHuman, Rat and Mouse liver homogenate lysates; HepG2 cells; Hela cells

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituent: 99% HBS
• Concentration information loading...
• Purification notesPurity is near homogeneity as judged by SDS-PAGE. ab129728 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
• Clonality Monoclonal
• Clone number5E2AD2
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

Applications

Target

• PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
• Involvement in diseaseDefects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
• Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.

Target information above from: UniProt accession P05062 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 229 Human
  • Entrez Gene: 230163 Mouse
  • Entrez Gene: 24190 Rat
  • Omim: 612724 Human
  • SwissProt: P05062 Human
  • SwissProt: Q91Y97 Mouse
  • SwissProt: P00884 Rat
  • Unigene: 530274 Human

  • Unigene: 482116 Mouse
  • Unigene: 98207 Rat

  see all

• Alternative names
  ALDB antibodyALDO B antibodyALDO2 antibody

  ALDOB antibodyALDOB_HUMAN antibodyAldolase 2 antibodyAldolase B antibodyAldolase B fructose bisphosphate antibodyAldolase2 antibodyAldolaseB antibodyEC 4.1.2.13 antibodyFructose bisphosphate aldolase B antibodyFructose-bisphosphate aldolase B antibodyLiver type aldolase antibodyLiver-type aldolase antibody

  see all

Anti-ALDOB antibody [5E2AD2] images

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  Immunoprecipitation - Anti-ALDOB antibody [5E2BD2] (ab129728)
  Immunoprecipitation image of ab129728. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain. Antibody ab129728 immunocaptured 39.4 kDa ALDOB only from rat liver homogenate (RLH), not from rat brain homogenate (RBH) nor rat skeletal muscle homogenate (RSMH). Gel stained with Coomassie brilliant blue G. Lane1: ladder Lane2: rat brain homogenate lysate Lane3: rat liver homogenate lysate Lane4: rat skeletal muscle homogenate lysate
• 

  Immunoprecipitation - Anti-ALDOB antibody [5E2BD2] (ab129728)
  Immunoprecipitation image of ab129728. ALDOB antibody immunocaptured 39.4 kDa ALDOB from human liver homogenate (HLH), Rat liver homogenate (RLH) and Mouse liver homogenate (MLH). Gel stained with Coomassie brilliant blue G.
  
  Lane 1: Ladder
  Lane 2: Human liver homogenate lysate
  Lane 3: Rat liver homogenate lysate
  Lane 4: Mouse liver homogenate lysate
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ALDOB antibody [5E2BD2] (ab129728)
  Immunocytochemistry image of ab129728 stained HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab129728 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Antigen retrieval step is recommended for a better signal. The target protein locates to the cytoplasm.
• 

  Flow Cytometry - Anti-ALDOB antibody [5E2BD2] (ab129728)
  Flow cytometry using ab129728. Hela cells were stained with 5 µg/mL ab129728 antibody (blue) or no primary antibody control (red) and analyzed by flow cytometry.