MitoSciences (MS1077)

Anti-ALDOB antibody [5E2AD2] (ab129728)

Overview

  • Product nameAnti-ALDOB antibody [5E2AD2]See all ALDOB primary antibodies ...
  • Description
    Mouse monoclonal [5E2AD2] to ALDOB
  • Tested applicationsIP, ICC/IF, In-Cell ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Purified Human liver mitochondria

  • Positive control
    • Human, Rat and Mouse liver homogenate lysates; HepG2 cells; Hela cells

Properties

Applications

Our Abpromise guarantee covers the use of ab129728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
In-Cell ELISA Use a concentration of 2 µg/ml.
Flow Cyt Use a concentration of 5 µg/ml.
  • Application notesIs unsuitable for WB.
  • Target

    • PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
    • Involvement in diseaseDefects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
    • Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALDB antibody
      • ALDO B antibody
      • ALDO2 antibody
      • ALDOB antibody
      • ALDOB_HUMAN antibody
      • Aldolase 2 antibody
      • Aldolase B antibody
      • Aldolase B fructose bisphosphate antibody
      • Aldolase2 antibody
      • AldolaseB antibody
      • EC 4.1.2.13 antibody
      • Fructose bisphosphate aldolase B antibody
      • Fructose-bisphosphate aldolase B antibody
      • Liver type aldolase antibody
      • Liver-type aldolase antibody
      see all

    Anti-ALDOB antibody [5E2AD2] images

    • Immunoprecipitation image of ab129728. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain. Antibody ab129728 immunocaptured 39.4 kDa ALDOB only from rat liver homogenate (RLH), not from rat brain homogenate (RBH) nor rat skeletal muscle homogenate (RSMH). Gel stained with Coomassie brilliant blue G. Lane1: ladder Lane2: rat brain homogenate lysate Lane3: rat liver homogenate lysate Lane4: rat skeletal muscle homogenate lysate
    • Immunoprecipitation image of ab129728. ALDOB antibody immunocaptured 39.4 kDa ALDOB from human liver homogenate (HLH), Rat liver homogenate (RLH) and Mouse liver homogenate (MLH). Gel stained with Coomassie brilliant blue G.
      Lane 1: Ladder
      Lane 2: Human liver homogenate lysate
      Lane 3: Rat liver homogenate lysate
      Lane 4: Mouse liver homogenate lysate
    • Immunocytochemistry image of ab129728 stained HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab129728 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Antigen retrieval step is recommended for a better signal. The target protein locates to the cytoplasm.
    • Flow cytometry using ab129728. Hela cells were stained with 5 µg/mL ab129728 antibody (blue) or no primary antibody control (red) and analyzed by flow cytometry.

    References for Anti-ALDOB antibody [5E2AD2] (ab129728)

    ab129728 has not yet been referenced specifically in any publications.

    Product Wall

    The antibody was tested and found to be negative in western blotting.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"