1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notesIs unsuitable for ICC or IP.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Involvement in diseaseDefects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.
ab75751 showing negative staining in Normal tonsil tissue.
References for Anti-ALDOB antibody [EPR3138Y] (ab75751)
This product has been referenced in:
Wang C et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One9:e101315 (2014).
Read more (PubMed: 24983750) »
Tun HW et al. Pathway signature and cellular differentiation in clear cell renal cell carcinoma. PLoS One5:e10696 (2010).
Read more (PubMed: 20502531) »