Overview

  • Product nameAnti-ALDOB antibody [EPR3138Y]
    See all ALDOB primary antibodies
  • Description
    Rabbit monoclonal [EPR3138Y] to ALDOB
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human ALDOB.

  • Positive control
    • Fetal kidney lysate. Human liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC or IP.
  • Target

    • PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
    • Involvement in diseaseDefects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
    • Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALDB antibody
      • ALDO B antibody
      • ALDO2 antibody
      • ALDOB antibody
      • ALDOB_HUMAN antibody
      • Aldolase 2 antibody
      • Aldolase B antibody
      • Aldolase B fructose bisphosphate antibody
      • Aldolase2 antibody
      • AldolaseB antibody
      • EC 4.1.2.13 antibody
      • Fructose bisphosphate aldolase B antibody
      • Fructose-bisphosphate aldolase B antibody
      • Liver type aldolase antibody
      • Liver-type aldolase antibody
      • MS1077 antibody
      see all

    Anti-ALDOB antibody [EPR3138Y] images

    • Anti-ALDOB antibody [EPR3138Y] (ab75751) at 1/1000 dilution + fetal kidney lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa
    • ab75751, at 1/100 dilution, staining ALDOB in paraffin-embedded human liver tissue.
    • ab75751 showing positive staining in Normal kidney tissue.

    • ab75751 showing negative staining in Normal colon tissue.

    • ab75751 showing negative staining in Normal lung tissue.

    • ab75751 showing negative staining in Normal tonsil tissue.

    References for Anti-ALDOB antibody [EPR3138Y] (ab75751)

    This product has been referenced in:
    • Wang C  et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One 9:e101315 (2014). WB . Read more (PubMed: 24983750) »
    • Tun HW  et al. Pathway signature and cellular differentiation in clear cell renal cell carcinoma. PLoS One 5:e10696 (2010). IHC-P ; Human . Read more (PubMed: 20502531) »

    See all 2 Publications for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"