Recombinant
RabMAb

Anti-Aldolase antibody [EPR9724(B)] (ab169544)

Overview

  • Product name
    Anti-Aldolase antibody [EPR9724(B)]
    See all Aldolase primary antibodies
  • Description
    Rabbit monoclonal [EPR9724(B)] to Aldolase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide within Human Aldolase aa 1-100. The exact sequence is proprietary.
    Database link: P04075

  • Positive control
    • WB: A549 and HeLa cell lysates; human fetal brain, fetal liver and skeletal muscle tissue lysates. IHC-P: Human muscle and ovarian carcinoma tissues. Flow Cyt: MCF-7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/230.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
    • Pathway
      Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
    • Involvement in disease
      Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
    • Sequence similarities
      Belongs to the class I fructose-bisphosphate aldolase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALDA antibody
      • Aldo1 antibody
      • ALDOA antibody
      • ALDOA_HUMAN antibody
      • Aldolase 1 antibody
      • Aldolase A antibody
      • Aldolase A fructose bisphosphatase antibody
      • Aldolase A fructose bisphosphate antibody
      • Aldolase, fructose-bisphosphate A antibody
      • Epididymis secretory sperm binding protein Li 87p antibody
      • FRUCTOALDOLASE A antibody
      • Fructose 1 6 bisphosphate triosephosphate lyase antibody
      • Fructose bisphosphate aldolase A antibody
      • Fructose bisphosphate aldolase antibody
      • FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A antibody
      • Fructose-bisphosphate aldolase A antibody
      • Fructose-bisphosphate aldolase A Muscle-type antibody
      • GSD12 antibody
      • HEL S 87p antibody
      • Lung cancer antigen NY LU 1 antibody
      • Lung cancer antigen NY-LU-1 antibody
      • MGC10942 antibody
      • MGC17716 antibody
      • MGC17767 antibody
      • Muscle type aldolase antibody
      • Muscle-type aldolase antibody
      • RNALDOG5 antibody
      see all

    Images

    • All lanes : Anti-Aldolase antibody [EPR9724(B)] (ab169544) at 1/1000 dilution

      Lane 1 : A549 lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : HeLa lysate
      Lane 4 : Fetal brain lysate
      Lane 5 : Human skeletal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Aldolase with ab169544 at 1/100 dilution.
    • Flow cytometry analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling Aldolase (red) with ab169544 at a 1/230 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling Aldolase with ab169544 at 1/100 dilution.

    References

    This product has been referenced in:
    • Zeng Y  et al. G6PC3, ALDOA and CS induction accompanies mir-122 down-regulation in the mechanical asphyxia and can serve as hypoxia biomarkers. Oncotarget 7:74526-74536 (2016). WB . Read more (PubMed: 27793029) »
    • Godar RJ  et al. Repetitive stimulation of autophagy-lysosome machinery by intermittent fasting preconditions the myocardium to ischemia-reperfusion injury. Autophagy 11:1537-60 (2015). WB ; Mouse . Read more (PubMed: 26103523) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (12.5% Acrylamide)
    Sample
    Pig Tissue lysate - other (Muscle Sarcoplasm)
    Specification
    Muscle Sarcoplasm
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Shannon Cruzen

    Verified customer

    Submitted Sep 29 2014

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Human Tissue lysate - whole (CNS tissue)
    Specification
    CNS tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 01 2014

    Application
    Western blot
    Loading amount
    75 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Mouse Tissue lysate - whole (Brain tissue)
    Specification
    Brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 13 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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