Recombinant Anti-Aldolase antibody [EPR9725] (ab150396)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9725] to Aldolase
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Aldolase antibody [EPR9725]
See all Aldolase primary antibodies -
Description
Rabbit monoclonal [EPR9725] to Aldolase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human Aldolase aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- MCF7, HepG2, and A549 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9725 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
Target
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Function
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein. -
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
Involvement in disease
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis. -
Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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Database links
- Entrez Gene: 226 Human
- Entrez Gene: 24189 Rat
- Omim: 103850 Human
- SwissProt: P04075 Human
- SwissProt: P05065 Rat
- Unigene: 513490 Human
- Unigene: 1774 Rat
- Unigene: 178420 Rat
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Alternative names
- ALDA antibody
- Aldo1 antibody
- ALDOA antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab150396 has been referenced in 1 publication.
- Xu C et al. Thrombospondin 2/Toll-Like Receptor 4 Axis Contributes to HIF-1a-Derived Glycolysis in Colorectal Cancer. Front Oncol 10:557730 (2020). PubMed: 33244454