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Western blot - Aldolase antibody (HRP) (ab34598)
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Product Name
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Aldolase antibody (HRP)
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See all Aldolase antibodies (11)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Aldolase (HRP)
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Immunogen
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Full length Aldolase protein (Rabbit muscle).
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Reacts with
(species key)
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Hu, Rb
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Specificity
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This antibody detects Aldolase.
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Tested applications
(see key)
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Dot, ELISA, EM, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: Use at an assay dependent dilution.
Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/2000.
WB: 1/1000. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB:Human A293, MCF7, NMB231 and HBL 100 cell lysates.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
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Purification notes
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This antibody was purified by a multi step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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10.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Aldolase antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Aldolase antibody (HRP) images, references, products related to ab34598 and other tools.
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Aldolase antibody (HRP) images:
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Search PubMed (MEDLINE) for references to Aldolase
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Aldolase antibody (HRP) - more information
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Customer reviews (feedback) regarding Aldolase antibody (HRP) |
Customer FAQs regarding Aldolase antibody (HRP) |
Protocols for Aldolase antibody (HRP) |
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Aldolase antibody (HRP) - related products:
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Aldolase antibody (HRP) - other tools:
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Contact Abcam with a Technical Enquiry about Aldolase antibody (HRP) |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - Aldolase antibody (HRP) (ab34598)