The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDot: Use at an assay dependent dilution.
Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/2000.
WB: 1/1000. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Involvement in diseaseDefects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.