Overview

  • Product name
  • Description
    Rabbit polyclonal to Aldolase B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Aldolase B aa 15-265.
    Database link: P05062

  • Positive control
    • 293T, A431, HeLa, Neuro2A, GL261, C8D30 and PC12 whole cell lysates; Hela cells; Human hepatoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Pathway
    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
  • Involvement in disease
    Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
  • Sequence similarities
    Belongs to the class I fructose-bisphosphate aldolase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALDB antibody
    • ALDO B antibody
    • ALDO2 antibody
    • ALDOB antibody
    • ALDOB_HUMAN antibody
    • Aldolase 2 antibody
    • Aldolase B antibody
    • Aldolase B fructose bisphosphate antibody
    • Aldolase2 antibody
    • AldolaseB antibody
    • EC 4.1.2.13 antibody
    • Fructose bisphosphate aldolase B antibody
    • Fructose-bisphosphate aldolase B antibody
    • Liver type aldolase antibody
    • Liver-type aldolase antibody
    • MS1077 antibody
    see all

Images

  • All lanes : Anti-Aldolase B antibody (ab153828) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Anti-Aldolase B antibody (ab153828) at 1/1000 dilution + PC12 whole cell lysate at 30 µg

    Predicted band size: 39 kDa



    10 % SDS-PAGE
  • All lanes : Anti-Aldolase B antibody (ab153828) at 1/1000 dilution

    Lane 1 : Neuro2A whole cell lysate
    Lane 2 : GL261 whole cell lysate
    Lane 3 : C8D30 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10 % SDS-PAGE
  • Immunofluorescence analysis of methanol fixed HeLa cells labeling Aldolase B with ab153828 at 1/500. Lower panel shows costaining with Hoechst 33342.

  • Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling ALDOB with ab153828 at 1/500.

References

ab153828 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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