Immunocytochemistry/ Immunofluorescence - Aldolase C antibody - Astrocyte Marker (ab87122)
ICC/IF image of ab87122 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87122, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 10µg/ml.
Immunohistochemistry (Frozen sections) - Aldolase C antibody - Astrocyte Marker (ab87122)Image courtesy of Lynette Foo, Stanford University, United States
IHC-Fr image of Aldolase C staining on Mouse cortex sections using ab87122 (1:1000). The sections were fixed in paraformaldehyde and permeabilized using 0.4% triton x. The sections were blcoked using 50% serum for 30 mins at 24°C.