Overview

  • Product nameAnti-ALG1 antibody
    See all ALG1 primary antibodies
  • Description
    Rabbit polyclonal to ALG1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cat, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 38-87 (VVLGDVGRSP RMQYHALSLA MHGFSVTLLG FCNSKPHDEL LQNNRIQIVG) of Human ALG1 (NP_061982).

  • Positive control
    • NTERA2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • FunctionParticipates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER.
  • PathwayProtein modification; protein glycosylation.
  • Involvement in diseaseDefects in ALG1 are the cause of congenital disorder of glycosylation type 1K (CDG1K) [MIM:608540]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
  • Sequence similaritiesBelongs to the glycosyltransferase 1 family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 4-mannosyltransferase antibody
    • Alg1 antibody
    • ALG1_HUMAN antibody
    • asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) antibody
    • asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) antibody
    • Asparagine-linked glycosylation protein 1 homolog antibody
    • Beta 1 4 mannosyltransferase antibody
    • Beta-1 antibody
    • CDG1K antibody
    • Chitobiosyldiphosphodolichol beta-mannosyltransferase antibody
    • GDP Man GlcNAc2 PP dolichol mannosyltransferase antibody
    • GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase antibody
    • GDP-mannose-dolichol diphosphochitobiose mannosyltransferase antibody
    • Hmat-1 antibody
    • HMAT1 antibody
    • HMT 1 antibody
    • HMT1 antibody
    • Mannosyltransferase 1 antibody
    • Mannosyltransferase-1 antibody
    • MT 1 antibody
    • MT-1 antibody
    • MT1 antibody
    see all

Anti-ALG1 antibody images

  • Anti-ALG1 antibody (ab81134) at 1 µg/ml (in 5% skim milk / PBS buffer) + NTERA2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa

References for Anti-ALG1 antibody (ab81134)

ab81134 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"