Overview

  • Product name
    Anti-ALG14 antibody
  • Description
    Rabbit polyclonal to ALG14
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ALG14 aa 73-102 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q96F25

  • Positive control
    • NCI-H460 cell line lysate, Human lung carcinoma and NCI-H460 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176239 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 24 kDa.

Target

Images

  • Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram), labeling ALG14, using ab176239 at a 1/10 dilution.  FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human lung carcinoma labeling ALG14 with ab176239 at a 1/50 dilution, followed by peroxidase-conjugation to the secondary antibody and DAB staining.

  • Anti-ALG14 antibody (ab176239) at 1/1000 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size : 24 kDa

References

ab176239 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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