The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
RelevanceALG6 (Asparagine-linked glycosylation 6 homolog) is a Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase which catalyzes the addition of the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. It transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man(9)GlcNAc(2)-PP-Dol.