Overview

  • Product nameAnti-ALIX antibody [3A9]
    See all ALIX primary antibodies
  • Description
    Mouse monoclonal [3A9] to ALIX
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Fusion protein corresponding to Human ALIX.

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab117600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 96 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Do not perform antigen retrieval.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionClass E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
  • Sequence similaritiesContains 1 BRO1 domain.
  • Cellular localizationCytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIP1 antibody
    • ALG 2 interacting protein 1 antibody
    • ALG-2-interacting protein 1 antibody
    • ALG2 interacting protein X antibody
    • Alix antibody
    • Apoptosis linked gene 2 interacting protein X antibody
    • Dopamine receptor interacting protein 4 antibody
    • DRIP4 antibody
    • Hp95 antibody
    • KIAA1375 antibody
    • MGC17003 antibody
    • PDC6I_HUMAN antibody
    • PDCD6 interacting protein antibody
    • PDCD6-interacting protein antibody
    • PDCD6IP antibody
    • Programmed cell death 6 interacting protein antibody
    • Programmed cell death 6-interacting protein antibody
    see all

Anti-ALIX antibody [3A9] images

  • Anti-ALIX antibody [3A9] (ab117600) + HeLa whole cell lysate

    Secondary
    Rabbit Anti Mouse IgG

    Predicted band size : 96 kDa

References for Anti-ALIX antibody [3A9] (ab117600)

This product has been referenced in:
  • Wiklander OP  et al. Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting. J Extracell Vesicles 4:26316 (2015). Read more (PubMed: 25899407) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Epethelial cells)
Specification Epethelial cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Verified customer

Submitted Aug 20 2014

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (Epethelial cells)
Specification Epethelial cells
Total protein in input 250 µg
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Submitted Aug 20 2014

Thank you for contacting us.

Here is the WB protocol you had requested.

Western Blotting

Reagents
1. Blocking Buffer
5% non-fat dried milk dissolved in PBS.
Note: For the detection of phosphoproteins, NEVE...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"