Overview

  • Product nameAnti-ALIX antibody
    See all ALIX primary antibodies
  • Description
    Rabbit polyclonal to ALIX
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to Human ALIX aa 400-450.

  • Positive control
    • HeLa whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab104256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionClass E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
    • Sequence similaritiesContains 1 BRO1 domain.
    • Cellular localizationCytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIP1 antibody
      • ALG 2 interacting protein 1 antibody
      • ALG-2-interacting protein 1 antibody
      • ALG2 interacting protein X antibody
      • Alix antibody
      • Apoptosis linked gene 2 interacting protein X antibody
      • Dopamine receptor interacting protein 4 antibody
      • DRIP4 antibody
      • Hp95 antibody
      • KIAA1375 antibody
      • MGC17003 antibody
      • PDC6I_HUMAN antibody
      • PDCD6 interacting protein antibody
      • PDCD6-interacting protein antibody
      • PDCD6IP antibody
      • Programmed cell death 6 interacting protein antibody
      • Programmed cell death 6-interacting protein antibody
      see all

    Anti-ALIX antibody images

    • Detection of PDC6I in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab104256 at 3 µg/mg lysate for IP (Lane 1). For WB detection an anti-PDC6I antibody which recognizes a downstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-ALIX antibody (ab104256)

    ab104256 has not yet been referenced specifically in any publications.

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