Overview

  • Product nameAnti-ALIX antibody
    See all ALIX primary antibodies
  • Description
    Mouse polyclonal to ALIX
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human ALIX.

  • Positive control
    • WB: lysate from Human liver or HeLa cells; ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionClass E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
  • Sequence similaritiesContains 1 BRO1 domain.
  • Cellular localizationCytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIP1 antibody
    • ALG 2 interacting protein 1 antibody
    • ALG-2-interacting protein 1 antibody
    • ALG2 interacting protein X antibody
    • Alix antibody
    • Apoptosis linked gene 2 interacting protein X antibody
    • Dopamine receptor interacting protein 4 antibody
    • DRIP4 antibody
    • Hp95 antibody
    • KIAA1375 antibody
    • MGC17003 antibody
    • PDC6I_HUMAN antibody
    • PDCD6 interacting protein antibody
    • PDCD6-interacting protein antibody
    • PDCD6IP antibody
    • Programmed cell death 6 interacting protein antibody
    • Programmed cell death 6-interacting protein antibody
    see all

Anti-ALIX antibody images

  • Anti-ALIX antibody (ab88743) at 1 µg/ml + Human liver lysate at 50 µg

    Predicted band size : 97 kDa
  • Anti-ALIX antibody (ab88743) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 97 kDa
  • All lanes : Anti-ALIX antibody (ab88743) at 1 µg/ml

    Lane 1 : PDC6I-transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 97 kDa
  • Immunofluorescence analysis of PDC6I expression in PFA-fixed, permeabilized HeLa cells using ab88743 at 10 µg/ml, followed by a fluorescein-conjugated secondary antibody.

References for Anti-ALIX antibody (ab88743)

This product has been referenced in:
  • Chakrabortty SK  et al. Extracellular vesicle-mediated transfer of processed and functional RNY5 RNA. RNA 21:1966-79 (2015). WB ; Human . Read more (PubMed: 26392588) »
  • Alvarez-Erviti L  et al. Lysosomal dysfunction increases exosome-mediated alpha-synuclein release and transmission. Neurobiol Dis 42:360-7 (2011). WB ; Human . Read more (PubMed: 21303699) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Leech (Hirudo medicinalis) Tissue lysate - whole (Central Nervous System)
Specification Central Nervous System
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"