Overview

  • Product nameAnti-ALK antibody [5A4]
    See all ALK primary antibodies
  • Description
    Mouse monoclonal [5A4] to ALK
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of NPM-ALK transcript (419-520 aa).

  • Positive control
    • Anaplastic lymphoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab17127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/70. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionNeuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
  • Tissue specificityExpressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
  • Involvement in diseaseA chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
    A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
    A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
    Neuroblastoma 3
    The ALK signaling pathway plays an important role in glioblastoma, the most common malignant brain tumor of adults and one of the most lethal cancers. It regulates both glioblastoma migration and growth.
    A chromosomal aberration involving ALK is found in one subject with colorectal cancer. Translocation t(2;2)(p23.1;p23.3). A 5 million base pair tandem duplication generates an in-frame WDCP-ALK gene fusion.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 LDL-receptor class A domain.
    Contains 2 MAM domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association.
    N-glycosylated.
  • Cellular localizationCell membrane. Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alk antibody
    • ALK tyrosine kinase receptor antibody
    • ALK/EML4 fusion gene, included antibody
    • ALK/NPM1 fusion gene, included antibody
    • ALK_HUMAN antibody
    • anaplastic lymphoma kinase (Ki-1) antibody
    • Anaplastic lymphoma kinase antibody
    • Anaplastic lymphoma kinase Ki1 antibody
    • anaplastic lymphoma receptor tyrosine kinase antibody
    • CD 246 antibody
    • CD246 antibody
    • CD246 antigen antibody
    • EC 2.7.10.1 antibody
    • Ki 1 antibody
    • Ki1 antibody
    • mutant anaplastic lymphoma kinase antibody
    • NBLST 3 antibody
    • NBLST3 antibody
    • Tcrz antibody
    • TFG/ALK antibody
    see all

Anti-ALK antibody [5A4] images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human anaplastic lymphoma tissue, staining ALK with ab17127.

References for Anti-ALK antibody [5A4] (ab17127)

This product has been referenced in:
  • Le Quesne J  et al. A comparison of immunohistochemical assays and FISH in detecting the ALK translocation in diagnostic histological and cytological lung tumor material. J Thorac Oncol 9:769-74 (2014). Read more (PubMed: 24787965) »
  • Ferretti GR  et al. Adequacy of CT-guided biopsies with histomolecular subtyping of pulmonary adenocarcinomas: Influence of ATS/ERS/IASLC guidelines. Lung Cancer 82:69-75 (2013). Read more (PubMed: 23927885) »

See all 5 Publications for this product

Product Wall



L'anticorps anti-ALK ab17127 a été testé en IHC-Paraffine et devrait fonctionner sans problème avec un automate Benchmark XT Ventana.
Veuillez noter que nous recommandons de procéder à un dé...

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Thank you for your enquiry and interest in our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datashee...

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Merci pour ces précisions qui nous permettent de prendre les dispositions nécessaires quant au lot de ab17127 défectueux. J'ai mis en place l'envoi d'une unité de ab17127 lot GR45198-2 en remplacement du lot GR28265-2. Vous recevrez prochaineme...

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Merci de nous avoir contactés. Comme expliqué par téléphone, nos produits sont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique (si le problème nous est communiqué dans les 6 mois qui suivent la commande). Nous pouvons d...

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Thank you for your enquiry. The immunogen sequence is spans the tyrosine kinase catalytic domain and part of the C-terminus of NPM-ALK transcript (419-520 aa). Please refer to the following publication for more information on the sequence. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"