Recombinant fragment: WQHQP EDRP NFAIIL ERI EYCTQDP DV INTALPIE Y GPLVEEEEK VPVRPKDPEG VPPLLVSQQ A KREEERSP AA PPPLPTT SSG KAAKKP TAAE VSVRV PRG, corresponding to amino acids 1366-1468 of Human ALK, a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.
This antibody recognises a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This rabbit polyclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.
Our Abpromise guarantee covers the use of ab16670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 176 kDa. PubMed: 21847362|
Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Immunohistochemical analysis of Human anaplastic lymphoma tissue labelling ALK with ab16670.
Immunohistochemical analysis of paraffin-embedded Human anaplastic lymphoma tissue labelling ALK with ab16670 at 1/100.