Anti-Alkaline Phosphatase antibody [4H1] (ab54778)


  • Product name
    Anti-Alkaline Phosphatase antibody [4H1]
    See all Alkaline Phosphatase primary antibodies
  • Description
    Mouse monoclonal [4H1] to Alkaline Phosphatase
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 91-200 of Human Alkaline Phosphatase (non tissue-specific)

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.



Our Abpromise guarantee covers the use of ab54778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.


  • Sequence similarities
    Belongs to the alkaline phosphatase family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alkaline phosphatase, germ cell antibody
    • Alkaline phosphatase, testicular and thymus antibody
    • Akp2 antibody
    • Alkaline phosphatase intestinal antibody
    • Alkaline phosphatase intestinal precursor antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental like 2 antibody
    • Alkaline phosphatase placental like antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase, liver antibody
    • Alkaline phosphatase, tissue-nonspecific antibody
    • Alkaline phosphomonoesterase antibody
    • ALP 1 antibody
    • ALP antibody
    • ALP I antibody
    • ALP L antibody
    • ALP P antibody
    • ALPG antibody
    • Alpi antibody
    • ALPL antibody
    • ALPP antibody
    • ALPPL antibody
    • ALPPL2 antibody
    • AP TNAP antibody
    • FLJ40094 antibody
    • GCAP antibody
    • Germ cell alkaline phosphatase antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • IAP antibody
    • Intestinal alkaline phosphatase antibody
    • Intestinal-type alkaline phosphatase antibody
    • Kasahara isozyme antibody
    • Nagao isozyme antibody
    • OTTHUMP00000164357 antibody
    • Placental alkaline phosphatase antibody
    • Placental like alkaline phosphatase antibody
    • PLAP 1 antibody
    • PLAP antibody
    • PLAP like antibody
    • PPBI_HUMAN antibody
    • Regan isozyme antibody
    • Testicular and thymus alkaline phosphatase antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all


  • IHC image of ab54778 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54778, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HepG2 cells stained with ab54778 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54778, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:

See all 4 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Frozen sections)
Cow Tissue sections (growth plate)
growth plate
fresh, no fixation
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 18 2013



Expiration date: 30-05-2013

Value: £235 each

I am very pleased to hear you would like to accept our offer and test ab54778 and ab128026 in Bovine. This code will g...

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Thank you for contacting us. Please contact our Indian distributor to buy these products. You can’t purchase products directly fromAbcam. The contact addressof Indian distributor is Allied Scientific Products 39/19B Gopal Nagar Road Kolkata-7000...

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Thanks a lot for your patience. I am afraid this antibody is very likely to cross react with both rabbit and rat species, and therefore may not be suitable for your purposes. I could check with my lab colleagues, if you wish to, if ab95...

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Thank you for contacting us. My colleagues from the lab informed me that the image was obtained using the Leica HRP compact polymer detection system which is an all in one mouse and rabbit detection system. For IHC staining amplification I would...

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