Overview

  • Product nameAnti-Alkaline Phosphatase antibody
    See all Alkaline Phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Alkaline Phosphatase
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli].
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Escherichia coli
    Does not react with: all Mammals
  • Immunogen

    Full length protein (E. coli).

Properties

Applications

Our Abpromise guarantee covers the use of ab354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000 - 1/40000.
IP Use at an assay dependent dilution.
ELISA 1/8000 - 1/40000.

Target

  • Sequence similaritiesBelongs to the alkaline phosphatase family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Alternative names
    • Alkaline phosphatase, germ cell antibody
    • Alkaline phosphatase, testicular and thymus antibody
    • Akp2 antibody
    • Alkaline phosphatase intestinal antibody
    • Alkaline phosphatase intestinal precursor antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental like 2 antibody
    • Alkaline phosphatase placental like antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase, liver antibody
    • Alkaline phosphatase, tissue-nonspecific antibody
    • Alkaline phosphomonoesterase antibody
    • ALP 1 antibody
    • ALP antibody
    • ALP I antibody
    • ALP L antibody
    • ALP P antibody
    • ALPG antibody
    • Alpi antibody
    • ALPL antibody
    • ALPP antibody
    • ALPPL antibody
    • ALPPL2 antibody
    • AP TNAP antibody
    • FLJ40094 antibody
    • GCAP antibody
    • Germ cell alkaline phosphatase antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • IAP antibody
    • Intestinal alkaline phosphatase antibody
    • Intestinal-type alkaline phosphatase antibody
    • Kasahara isozyme antibody
    • Nagao isozyme antibody
    • OTTHUMP00000164357 antibody
    • Placental alkaline phosphatase antibody
    • Placental like alkaline phosphatase antibody
    • PLAP 1 antibody
    • PLAP antibody
    • PLAP like antibody
    • PPBI_HUMAN antibody
    • Regan isozyme antibody
    • Testicular and thymus alkaline phosphatase antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Anti-Alkaline Phosphatase antibody images

  • Ab354 staining Alkaline Phosphatase in E.coli recombinant protein Mach1 by indirect ELISA. Samples were blocked with 5%milk for 3 hours at room temperature and incubated with primary antibody (1/15000 in TBST + 5% Milk) for 90 minutes. An HRP-conjugated goat polyclonal to Rabbit IgG (1/30000) was used as secondary antibody.

    See Abreview

  • All lanes : Anti-Alkaline Phosphatase antibody (ab354) at 1/8000 dilution

    Lane 1 : Escherichia coli Recombinant protein (Mach1)
    Lane 2 : Escherichia coli Recombinant protein (Mach1)

    Lysates/proteins at 0.5 µg per lane.

    Secondary
    An HRP-conjugated anti-rabbit IgG (whole molecule) at 1/16000 dilution
    Developed using the ECL technique

    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 seconds

    Image courtesy of an anonymous Abreview.

References for Anti-Alkaline Phosphatase antibody (ab354)

This product has been referenced in:
  • Bross P  et al. The HSP60-(P.val98ile) mutation associated with hereditary spastic paraplegia SPG13 compromises chaperonin function both in vitro and in vivo. J Biol Chem : (2008). WB ; Escherichia coli . Read more (PubMed: 18400758) »
  • Imperi F  et al. Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa. Microbiology 154:2804-13 (2008). Read more (PubMed: 18757814) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Escherichia coli Recombinant protein (Mach1)
Specification Mach1
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type Indirect
Username

Abcam user community

Verified customer

Submitted Feb 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Recombinant protein (Mach1)
Loading amount 0.5 µg
Specification Mach1
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 19 2010

Thank you for your enquiry. This product is sold as tissue culture supernatant and not affinity purified. As a tissue culture supernatant this product will contain additional proteins and the IgG concentration has not been determined. Typically IgG con...

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This antibody should detect SEAP from E.coli. It has not been tested for cross-reactivity in other bacteria and does not cross-react with AP from mammalian species.

A working dilution from our test using lot 4679(assayed againest 1.0 ug of Alkaline Phosphatase in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anit- Rabbit IgG, was 1:8,000 to 1:40,000 (volume:volume).

No this antibody is not suitable as it does not cross react with the mammalian form of Alkaline Phosphatase. The immunogen is Alkaline Phosphatase from E.coli. We suggest you review our other alkaline phosphatase antibodies, many of which have been tes...

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No, this antibody is not a marker of plasma membrane.

We have no reports of it being used in this application, but do not see a reason why it should not recognise SEAP.

We believe it is although it might be more highly expressed in stationary phase and be strain dependent. Abcam

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"