Anti-Alkaline Phosphatase antibody (ab95462)

Overview

  • Product nameAnti-Alkaline Phosphatase antibody
    See all Alkaline Phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Alkaline Phosphatase
  • SpecificityThe immunogen is from the AP-TNAP protein (SwissProt P08289) and presents no homology with intestinal isoform of rat alkaline phosphatase.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, a wide range of other species
  • Immunogen

    Synthetic peptide derived from an internal domain of Rat Alkaline Phosphatase (Uniprot: P08289)

Properties

Applications

Our Abpromise guarantee covers the use of ab95462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/5000. Predicted molecular weight: 57 kDa.

Target

  • Sequence similaritiesBelongs to the alkaline phosphatase family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alkaline phosphatase, germ cell antibody
    • Alkaline phosphatase, testicular and thymus antibody
    • Akp2 antibody
    • Alkaline phosphatase intestinal antibody
    • Alkaline phosphatase intestinal precursor antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental like 2 antibody
    • Alkaline phosphatase placental like antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase, liver antibody
    • Alkaline phosphatase, tissue-nonspecific antibody
    • Alkaline phosphomonoesterase antibody
    • ALP 1 antibody
    • ALP antibody
    • ALP I antibody
    • ALP L antibody
    • ALP P antibody
    • ALPG antibody
    • Alpi antibody
    • ALPL antibody
    • ALPP antibody
    • ALPPL antibody
    • ALPPL2 antibody
    • AP TNAP antibody
    • FLJ40094 antibody
    • GCAP antibody
    • Germ cell alkaline phosphatase antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • IAP antibody
    • Intestinal alkaline phosphatase antibody
    • Intestinal-type alkaline phosphatase antibody
    • Kasahara isozyme antibody
    • Nagao isozyme antibody
    • OTTHUMP00000164357 antibody
    • Placental alkaline phosphatase antibody
    • Placental like alkaline phosphatase antibody
    • PLAP 1 antibody
    • PLAP antibody
    • PLAP like antibody
    • PPBI_HUMAN antibody
    • Regan isozyme antibody
    • Testicular and thymus alkaline phosphatase antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Anti-Alkaline Phosphatase antibody images

  • ab95462 staining alkaline phosphatase in Rat Kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.25% casein in PBS for 5 minutes at 25°C; antigen retrieval was by heat mediation with a Tris/EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/400 in antibody diluent) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Alkaline Phosphatase antibody (ab95462)

This product has been referenced in:
  • Wang F  et al. PTH/SDF-1a cotherapy induces CD90+CD34- stromal cells migration and promotes tissue regeneration in a rat periodontal defect model. Sci Rep 6:30403 (2016). IHC ; Rat . Read more (PubMed: 27480134) »
  • Qu X  et al. MiR-132-3p Regulates the Osteogenic Differentiation of Thoracic Ligamentum Flavum Cells by Inhibiting Multiple Osteogenesis-Related Genes. Int J Mol Sci 17:N/A (2016). WB ; Human . Read more (PubMed: 27556448) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (gel 10%)
Sample Mouse Cell lysate - whole cell (MC3T3-E1 cells)
Specification MC3T3-E1 cells
Treatment βGP+VitC, βGP+VitC+2.5 ´M DAPT for 3d, βGP+VitC+5 ´M DAPT for 3d, βGP+VitC+10 ´M DAPT for 3d
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Xiangyu Huang

Verified customer

Submitted Mar 30 2015

I am very pleased to hear you would like to accept our offer and test ab95462 in . This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for and include this code in the “Addit...

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Thank you for contacting us. Please contact our Indian distributor to buy these products. You can’t purchase products directly fromAbcam. The contact addressof Indian distributor is Allied Scientific Products 39/19B Gopal Nagar Road Kolkata-7000...

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Vielen Dank für Ihre Anfrage. Das Immunogen, das zur Herstellung von ab95462 verwendet wird, ist ein synthetisches Peptid, das von der Alkalischen Phosphatase der Ratte mit der SwissProt-ID P08289 abgeleitet ist. Leider weist dieses Peptid keine Seq...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney and Jejunum)
Specification Kidney and Jejunum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 21 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 21 2011

Thank you for your enquiry. I can confirm that ab95462 Alkaline Phosphatase antibody was raised against the following protein: Alkaline phosphatase, tissue-nonspecific isozyme precursor (EC 3.1.3.1) (AP-TNAP) (TNSALP) (Alkaline phosphataseliver...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta and Kidney)
Specification Placenta and Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jun 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Kidney)
Specification Kidney
Fixative 10% NBF
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS/EDTA pH 9.0
Permeabilization No
Blocking step Casein in PBS as blocking agent for 5 minute(s) · Concentration: 0.25% · Temperature: 25°C
Username

Ms. Elizabeth Chlipala

Verified customer

Submitted Nov 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"