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Abcam's Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369) is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure alkaline phosphatase (ALP) activity in serum and biological samples. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax= 405 nm) when dephosphorylated by ALP.
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This kit contains 10 substrate tablets providing convenience for multiple usages.
|ALP Assay buffer||NM||1 x 100ml|
|ALP Enzyme (Lyophilised)||Green||1 vial|
|pNPP Substrate (10 tablets)||Red||1 vial|
|Stop Solution||WM||1 x 10ml|
Our Abpromise guarantee covers the use of ab83369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Alkaline phosphatase was measured in Caco-2 cells after 48 hours treatment with PMA and Butyrate using Alkaline Phosphatase assay kit (ab83369).
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
ALP measured in cell culture supernatants showing activity (U) per L of tested sample. Samples were diluted 1-16 fold.
ALP measured in biological fluids showing activity (U) per L of tested sample. Samples were diluted 4-64 fold.
Measurement of ALP activity using ab83369. a. ALP activity in fresh medium (80 μL, without culturing), 3 day old HeLa cell culture medium (80 μL) and human serum (80 μL, 1/10 dilution). b. ALP activity in HeLa cells. 5 x 104 HeLa cells were homogenized in 1 mL of Assay Buffer, diluted 1/10 in Assay Buffer, and 80 μL used for the measurement.
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