Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (ab126820)

Overview

  • Product name
    Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4]
    See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies
  • Description
    Mouse monoclonal [2F4] to Alkaline Phosphatase, Tissue Non-Specific
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Alkaline Phosphatase, Tissue Non-Specific. expressed in E.coli

  • Positive control
    • Recombinant Human ALPL protein (AA: 18-502), HeLa and NTERA-2 cell lysates. This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: HepG2
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR307490-5 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab126820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 57.3, 78.9 kDa (predicted molecular weight: 57, 78.9 kDa).
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.

Target

  • Function
    This isozyme may play a role in skeletal mineralization.
  • Involvement in disease
    Hypophosphatasia
    Hypophosphatasia childhood type
    Hypophosphatasia infantile type
  • Sequence similarities
    Belongs to the alkaline phosphatase family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKP2 antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Images

  • ICC/IF image of ab126820 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126820 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) hepG2 cells used at 1/100 dilution.

  • Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (ab126820) at 1/500 dilution + Recombinant Human protein ALPL (AA: 18-502)

    Predicted band size : 57, 78.9 kDa
  • All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (ab126820) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : NTERA-2 cell lysate


    Predicted band size : 57, 78.9 kDa
  • Example of standard curves obtained using ab126820. Control antigen 100ng (black), antigen at 10ng (purple), antigen at 50ng (blue), antigen at 100ng (red)
  • Flow cytometric analysis of MCF-7 cells using ab126820 at 1/200 (green) and negative control (red).
  • ab126820, at 1/200, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human ovarian cancer tissues by Immunohistochemistry with DAB staining.

References

ab126820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



Below is the recommended flow cytometry protocol for use with ab126820:



Flow Cytometry Protocol

1. Solutions and Reagents

1.1. 1X PBS

1.2. Blocking buffer: 0.5% BSA in 1X PBS

1.3. F...

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