Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab65834)


  • Product nameAnti-Alkaline Phosphatase, Tissue Non-Specific antibody
    See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies
  • Description
    Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the N-terminus of Human Alkaline Phosphatase

  • Positive control
    • HeLa cells. Small intestine.



Our Abpromise guarantee covers the use of ab65834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20634292
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P Use at an assay dependent concentration.


  • FunctionThis isozyme may play a role in skeletal mineralization.
  • Involvement in diseaseHypophosphatasia
    Hypophosphatasia childhood type
    Hypophosphatasia infantile type
  • Sequence similaritiesBelongs to the alkaline phosphatase family.
  • Post-translational
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKP2 antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody images

  • This image shows ab65834 at 1-2µg/ml staining cytoplasmic Alkaline Phosphatase in rat small intestine tissue.
  • Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab65834) at 2 µg/ml + HeLa cell lysate

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • ICC/IF image of ab65834 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65834, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab65834)

This product has been referenced in:
  • Davis H  et al. Aberrant epithelial GREM1 expression initiates colonic tumorigenesis from cells outside the stem cell niche. Nat Med 21:62-70 (2015). Read more (PubMed: 25419707) »
  • Davis H  et al. Investigation of the atypical FBXW7 mutation spectrum in human tumours by conditional expression of a heterozygous propellor tip missense allele in the mouse intestines. Gut N/A:N/A (2013). IHC-P ; Mouse . Read more (PubMed: 23676439) »

See all 4 Publications for this product

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Although the exact immnuogen sequences are considered to be proprietary, I can let you the following information narrowing down the regions that these antibodies recognize:
- ab108337 - Between ***
- ab658...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH6/8
Permeabilization No
Blocking step Serum Free Protein Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 21°C

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Submitted Jun 13 2011