Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab86789)
Key features and details
- Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Alkaline Phosphatase, Tissue Non-Specific antibody
See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies -
Description
Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Alkaline Phosphatase, Tissue Non-Specific aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab107105) -
Positive control
- This antibody gave a positive signal in Human kidney tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
This isozyme may play a role in skeletal mineralization. -
Involvement in disease
Hypophosphatasia
Hypophosphatasia childhood type
Hypophosphatasia infantile type -
Sequence similarities
Belongs to the alkaline phosphatase family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 249 Human
- Omim: 171760 Human
- SwissProt: P05186 Human
- Unigene: 75431 Human
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Alternative names
- AKP2 antibody
- Alkaline phosphatase liver/bone/kidney antibody
- Alkaline phosphatase liver/bone/kidney isozyme antibody
see all
Images
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Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab86789) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab86789 has not yet been referenced specifically in any publications.