Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab97384)

Overview

  • Product name
    Anti-Alkaline Phosphatase, Tissue Non-Specific antibody
    See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies
  • Description
    Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 1 and 369 of Human Alkaline Phosphatase, Tissue Non-Specific (NP_000469).

  • Positive control
    • Hela, HepG2, MOLT4 and Raji cell lines; OVCAR3 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab97384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    This isozyme may play a role in skeletal mineralization.
  • Involvement in disease
    Hypophosphatasia
    Hypophosphatasia childhood type
    Hypophosphatasia infantile type
  • Sequence similarities
    Belongs to the alkaline phosphatase family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKP2 antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Images

  • Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab97384) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 57 kDa
  • Immunohistochemical analysis of Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded OVCAR3 xenograft, using ab97384 at a 1/100 dilution.

References

ab97384 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 15µg/mL · Temperature: 25°C
Username

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Verified customer

Submitted Apr 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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