Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)
- Datasheet
- Specific References (5)
- Protocols
Overview
- Product nameAnti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E]
See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies - DescriptionMouse monoclonal [TRA-2-49/6E] to Alkaline Phosphatase, Tissue Non-Specific
- SpecificityThe clone number has been updated from (3H414 (TRA-2-49)) to (TRA-2-49/6E) both clone numbers name the same antibody clone.
- Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, IPmore details
- Species reactivityReacts with: Rabbit, Cat, Human, Pig, Chimpanzee, Gorilla, Hoolock gibbon, Orangutan, Owl monkey
Does not react with: Mouse, Rat, Sheep, Goat, Guinea pig, Hamster, Cow, Dog, Baboon, Rhesus monkey - Immunogen
2102Ep Human Embryonal Carcinoma Cells
- General notesClone 3H414 was previously known as TRA-2-49/6E.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.6 -
Concentration information loading... - PurityProtein A purified
- ClonalityMonoclonal
- Clone numberTRA-2-49/6E
- IsotypeIgG1
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab17973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/50. PubMed: 23225236 | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | 1/50 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
| IP | Use at an assay dependent concentration. |
Target
- FunctionThis isozyme may play a role in skeletal mineralization.
- Involvement in diseaseHypophosphatasia
Hypophosphatasia childhood type
Hypophosphatasia infantile type - Sequence similaritiesBelongs to the alkaline phosphatase family.
- Post-translational
modificationsN-glycosylated. - Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 249 Human
- Omim: 171760 Human
- SwissProt: Q29486 Cat
- SwissProt: P05186 Human
- Unigene: 75431 Human
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Alternative names
- AKP2 antibody
- Alkaline phosphatase liver/bone/kidney antibody
- Alkaline phosphatase liver/bone/kidney isozyme antibody
see all
Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] images
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![Immunocytochemistry/ Immunofluorescence - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)](http://a.abcam.com/ps/products/17/ab17973/Images/ab17973_11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)This image is courtesy of Ludovic Vallier, University of CambridgeUndifferentiated human embryonic stem cells (H9 line) were stained with the tissue non-specific alkaline phosphatase antibody, ab17973. The cells were positive for alkaline phosphatase with staining localised on the surface membrane.
Dilution used: 1/50
Note that the feeder cells in the bottom left hand corner of the plate are not stained by this antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)](http://a.abcam.com/ps/products/17/ab17973/Images/Alkaline-Phosphatase-Tissue-Non-Specific-Primary-antibodies-ab17973-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)This image is courtesy of an anonymous abreview.ab17973 staining Alkaline Phosphatase, Tissue Non-Specific in human adrenal gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated rabbit anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.
References for Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)
This product has been referenced in:
- Alves RD et al. Calcifying vascular smooth muscle cells and osteoblasts: independent cell types exhibiting extracellular matrix and biomineralization-related mimicries. BMC Genomics 15:965 (2014). Flow Cyt ; Human . Read more (PubMed: 25380738) »
- Aliagas E et al. Ecto-nucleotidases distribution in human cyclic and postmenopausic endometrium. Purinergic Signal 9:227-37 (2013). IHC-Fr ; Human . Read more (PubMed: 23225236) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"