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2102Ep Human Embryonal Carcinoma Cells
Clone 3H414 was previously known as TRA-2-49/6E.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab218574 as a replacement.
Our Abpromise guarantee covers the use of ab17973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/50. PubMed: 23225236|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||1/50 - 1/200.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
Undifferentiated human embryonic stem cells (H9 line) were stained with the tissue non-specific alkaline phosphatase antibody, ab17973. The cells were positive for alkaline phosphatase with staining localised on the surface membrane.
Dilution used: 1/50
Note that the feeder cells in the bottom left hand corner of the plate are not stained by this antibody.