Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E]
See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies -
DescriptionMouse monoclonal [TRA-2-49/6E] to Alkaline Phosphatase, Tissue Non-Specific
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Host speciesMouse
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SpecificityThe clone number has been updated from (3H414 (TRA-2-49)) to (TRA-2-49/6E) both clone numbers name the same antibody clone.
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Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, IPmore details
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Species reactivityReacts with: Rabbit, Cat, Human, Pig, Chimpanzee, Gorilla, Hoolock gibbon, Orangutan, Owl monkey
Does not react with: Mouse, Rat, Sheep, Goat, Guinea pig, Hamster, Cow, Dog, Baboon, Rhesus monkey -
Immunogen
2102Ep Human Embryonal Carcinoma Cells
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General notes
Clone 3H414 was previously known as TRA-2-49/6E.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab218574 as a replacement.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.6 -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberTRA-2-49/6E
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IsotypeIgG1
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab17973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/50. PubMed: 23225236 | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | 1/50 - 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IP | Use at an assay dependent concentration. |
Target
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FunctionThis isozyme may play a role in skeletal mineralization.
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Involvement in diseaseHypophosphatasia
Hypophosphatasia childhood type
Hypophosphatasia infantile type -
Sequence similaritiesBelongs to the alkaline phosphatase family.
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Post-translational
modificationsN-glycosylated. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 727689 Cat
- Entrez Gene: 249 Human
- Omim: 171760 Human
- SwissProt: Q29486 Cat
- SwissProt: P05186 Human
- Unigene: 75431 Human
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Alternative names
- AKP2 antibody
- Alkaline phosphatase liver/bone/kidney antibody
- Alkaline phosphatase liver/bone/kidney isozyme antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)](http://a.abcam.com/ps/products/17/ab17973/Images/ab17973_11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)This image is courtesy of Ludovic Vallier, University of CambridgeUndifferentiated human embryonic stem cells (H9 line) were stained with the tissue non-specific alkaline phosphatase antibody, ab17973. The cells were positive for alkaline phosphatase with staining localised on the surface membrane.
Dilution used: 1/50
Note that the feeder cells in the bottom left hand corner of the plate are not stained by this antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)](http://a.abcam.com/ps/products/17/ab17973/Images/Alkaline-Phosphatase-Tissue-Non-Specific-Primary-antibodies-ab17973-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)This image is courtesy of an anonymous abreview.ab17973 staining Alkaline Phosphatase, Tissue Non-Specific in human adrenal gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated rabbit anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.
Protocols
References
This product has been referenced in:
- Alves RD et al. Calcifying vascular smooth muscle cells and osteoblasts: independent cell types exhibiting extracellular matrix and biomineralization-related mimicries. BMC Genomics 15:965 (2014). Flow Cyt ; Human . Read more (PubMed: 25380738) »
- Aliagas E et al. Ecto-nucleotidases distribution in human cyclic and postmenopausic endometrium. Purinergic Signal 9:227-37 (2013). IHC-Fr ; Human . Read more (PubMed: 23225236) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"