Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)


  • Product nameAnti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E]
    See all Alkaline Phosphatase, Tissue Non-Specific primary antibodies
  • Description
    Mouse monoclonal [TRA-2-49/6E] to Alkaline Phosphatase, Tissue Non-Specific
  • SpecificityThe clone number has been updated from (3H414 (TRA-2-49)) to (TRA-2-49/6E) both clone numbers name the same antibody clone.
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Rabbit, Cat, Human, Pig, Chimpanzee, Gorilla, Hoolock gibbon, Orangutan, Owl monkey
    Does not react with: Mouse, Rat, Sheep, Goat, Guinea pig, Hamster, Cow, Dog, Baboon, Rhesus monkey
  • Immunogen

    2102Ep Human Embryonal Carcinoma Cells

  • General notesClone 3H414 was previously known as TRA-2-49/6E.



Our Abpromise guarantee covers the use of ab17973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50. PubMed: 23225236
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.


  • FunctionThis isozyme may play a role in skeletal mineralization.
  • Involvement in diseaseHypophosphatasia
    Hypophosphatasia childhood type
    Hypophosphatasia infantile type
  • Sequence similaritiesBelongs to the alkaline phosphatase family.
  • Post-translational
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKP2 antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] images

  • Undifferentiated human embryonic stem cells (H9 line) were stained with the tissue non-specific alkaline phosphatase antibody, ab17973. The cells were positive for alkaline phosphatase with staining localised on the surface membrane.

    Dilution used: 1/50

    Note that the feeder cells in the bottom left hand corner of the plate are not stained by this antibody.

  • ab17973 staining Alkaline Phosphatase, Tissue Non-Specific in human adrenal gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated rabbit anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

References for Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [TRA-2-49/6E] (ab17973)

This product has been referenced in:
  • Alves RD  et al. Calcifying vascular smooth muscle cells and osteoblasts: independent cell types exhibiting extracellular matrix and biomineralization-related mimicries. BMC Genomics 15:965 (2014). Flow Cyt ; Human . Read more (PubMed: 25380738) »
  • Aliagas E  et al. Ecto-nucleotidases distribution in human cyclic and postmenopausic endometrium. Purinergic Signal 9:227-37 (2013). IHC-Fr ; Human . Read more (PubMed: 23225236) »

See all 5 Publications for this product

Product Wall

The lab has tested ab17973 with H9 cells, NTERA-2 cl.D1 cells, iPSCs and A431 cells, while ab17272 has been tested with U2-OS cells. So, any of these cells can be used as positive controls.

Ab17973 was tested in the lab using the following pro...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Adrenal gland)
Specification Adrenal gland
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 01 2011

Thank you for your enquiry and your patience. I'm afraid this antibody is not specific for leukocyte alkaline phosphatase. It is only known to react with the human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP). Also, this antibody does not cro...

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