RabMAb

Anti-Alkaline Phosphatase, Tissue Non-Specific [EPR4477] antibody (ab108337)

Overview

  • Product nameAnti-Alkaline Phosphatase, Tissue Non-Specific [EPR4477] antibodySee all Alkaline Phosphatase, Tissue Non-Specific primary antibodies ...
  • Description
    Rabbit monoclonal [EPR4477] to Alkaline Phosphatase, Tissue Non-Specific
  • Tested applicationsFlow Cyt, WB, IP, IHC-P, ICC more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Alkaline Phosphatase, Tissue Non-Specific aa 1-100.

  • Positive control
    • WB: SAOS2, HepG2, HeLa, and JAR cell lysates IHC-P: Human kidney and liver tissues ICC/IF: HeLa cells
  • General notesThis product was manufactured under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab108337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt 1/1000.
WB 1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/500.

Target

  • FunctionThis isozyme may play a role in skeletal mineralization.
  • Involvement in diseaseDefects in ALPL are a cause of hypophosphatasia (HOPS) [MIM:146300]. HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto).
    Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC) [MIM:241510].
    Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI) [MIM:241500].
  • Sequence similaritiesBelongs to the alkaline phosphatase family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationCell membrane.
  • Target information above from: UniProt accession P05186 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AKP2 antibody
    • Alkaline phosphatase antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney isozyme antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • tissue-nonspecific isozyme antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Anti-Alkaline Phosphatase, Tissue Non-Specific [EPR4477] antibody images

  • Overlay histogram showing Saos 2 cells stained with ab108337 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108337, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Saos 2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific [EPR4477] antibody (ab108337) at 1/10000 dilution

    Lane 1 : SAOS2 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 57 kDa
  • ab108337, at a 1/250 dilution, staining Bone Alkaline Phosphatase in paraffin embedded Human kidney tissue by Immunohistochemistry.
  • ab108337, at a 1/250 dilution, staining Bone Alkaline Phosphatase in paraffin embedded Human liver tissue by Immunohistochemistry.
  • ab108337, at a 1/100 dilution, staining Bone Alkaline Phosphatase in HeLa cells by Immunofluorescence.

References for Anti-Alkaline Phosphatase, Tissue Non-Specific [EPR4477] antibody (ab108337)

This product has been referenced in:
  • Strauss S  et al. Adhesion, vitality and osteogenic differentiation capacity of adipose derived stem cells seeded on nitinol nanoparticle coatings. PLoS One 8:e53309 (2013). ICC/IF ; Human . Read more (PubMed: 23308190) »

See 1 Publication for this product

Product Wall

Thank you very much for your interest in ab108337.

To our knowledge, ab108337 has not been tested in flow cytometry. We do have four antibodies against bone alkaline phosphatase. Only the ab108337 has been tested against the rat protein and ...

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Thank you for contacting us.

This is correct. You will receive a vial of 100 ul with xxxxx ug of ab108337 antibody in it. This may appear like a small amount but this is because it is a rabbit monoclonal antibody which has a high affinity fo...

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Thank you for contacting us.

The concentration for Anti-Bone Alkaline Phosphatase antibody [EPR4477] (ab108337), lot GR79855-5 in stock is xxxxxx mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact ...

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Thank you for contacting Abcam.
Although the exact immnuogen sequences are considered to be proprietary, I can let you the following information narrowing down the regions that these antibodies recognize:
- ab108337 - Between ***
- ab658...

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Thank you for your enquiry and interest in our products.

I can confirm that both antibodies (ab22552 and ab108337) have been tested in IHC-P and recognize the murine proteins (Osterix and Bone Alkaline Phosphatase, respectively). This means t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2-OS)
Loading amount 25 µg
Specification U2-OS
Gel Running Conditions Reduced Denaturing (15%)
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

Vielen Dank für Ihre Anfrage.


Leider muss ich Sie bezüglich der Konzentration enttäuschen: Es handelt sich hier um einen unaufgreinigten Zellkulturüberstand, bei dem es unmöglich ist, die eigentliche Antikö...

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Thats no problem.
As I mentioned in my previous email, if you do have any further problems or questions, please do not hesitate to contact us again.
Until then, I wish you all the best with your research.

Thank you for contacting us and reporting the problem observed with the delivery of anti-Bone Alkaline Phosphatase antibody [EPR4477] (ab108337).
As discussed over the phone, we have carried out experiments in-house to assess the effect on tempera...

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Here is information regarding one of the bone alkaline phosphatase antibodies we discussed:
ab108337 has not been tested for its ability to recognize phosphorylated forms of the protein. We also have not tested bone samples.
I hope this is he...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"