Overview

  • Product name
  • Description
    Mouse polyclonal to ALKBH1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human ALKBH1.

  • Positive control
    • Jurkat cell lysate; ABH1 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab88775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.

Target

  • Function
    Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localization
    Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABH antibody
    • ABH1 antibody
    • alkB antibody
    • alkB homolog 1 histone H2A dioxygenase antibody
    • AlkB, alkylation repair homolog 1 (E. coli) antibody
    • ALKB1_HUMAN antibody
    • ALKBH antibody
    • ALKBH1 antibody
    • Alkylated DNA repair protein alkB homolog 1 antibody
    • Alkylation repair homolog 1 antibody
    • Alkylation repair, alkB homolog antibody
    • Alpha-ketoglutarate-dependent dioxygenase ABH1 antibody
    • DNA lyase ABH1 antibody
    • DNA oxidative demethylase ALKBH1 antibody
    • hABH antibody
    see all

Images

  • Anti-ALKBH1 antibody (ab88775) at 1/500 dilution + Jurkat cell lysate at 50 µg

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • All lanes : Anti-ALKBH1 antibody (ab88775) at 1/500 dilution

    Lane 1 : ALKBH transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 43 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References

ab88775 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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