Overview

  • Product name
  • Description
    Rabbit polyclonal to ALKBH1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human ALKBH1 aa 166-357.

  • Positive control
    • HeLa cell lysate or NT2D1 whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.

Target

  • Function
    Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localization
    Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABH antibody
    • ABH1 antibody
    • alkB antibody
    • alkB homolog 1 histone H2A dioxygenase antibody
    • AlkB, alkylation repair homolog 1 (E. coli) antibody
    • ALKB1_HUMAN antibody
    • ALKBH antibody
    • ALKBH1 antibody
    • Alkylated DNA repair protein alkB homolog 1 antibody
    • Alkylation repair homolog 1 antibody
    • Alkylation repair, alkB homolog antibody
    • Alpha-ketoglutarate-dependent dioxygenase ABH1 antibody
    • DNA lyase ABH1 antibody
    • DNA oxidative demethylase ALKBH1 antibody
    • hABH antibody
    see all

Images

  • Anti-ALKBH1 antibody (ab97589) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size : 44 kDa
    10% SDS PAGE

References

ab97589 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab97589.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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