Recombinant
RabMAb

Anti-ALKBH1 antibody [EPR19215] (ab195376)

Overview

  • Product name
    Anti-ALKBH1 antibody [EPR19215]
    See all ALKBH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR19215] to ALKBH1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Goat, Horse, Guinea pig, Cow, Cat, Dog, Non human primates
  • Immunogen

    Recombinant full length protein within Human ALKBH1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13686

  • Positive control
    • WB: Jurkat, K562, A549, A431, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; human fetal kidney, colon and fetal liver lysates; mouse brain and spleen lysates; rat spleen lysate. IP: K562 whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR19215
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

Milk recommended as blocking agent.

IP 1/30.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localization
      Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABH antibody
      • ABH1 antibody
      • alkB antibody
      • alkB homolog 1 histone H2A dioxygenase antibody
      • AlkB, alkylation repair homolog 1 (E. coli) antibody
      • ALKB1_HUMAN antibody
      • ALKBH antibody
      • ALKBH1 antibody
      • Alkylated DNA repair protein alkB homolog 1 antibody
      • Alkylation repair homolog 1 antibody
      • Alkylation repair, alkB homolog antibody
      • Alpha-ketoglutarate-dependent dioxygenase ABH1 antibody
      • DNA lyase ABH1 antibody
      • DNA oxidative demethylase ALKBH1 antibody
      • hABH antibody
      see all

    Images

    • All lanes : Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution

      Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
      Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 44 kDa
      Observed band size: 44 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human colon lysate
      Lane 3 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 44 kDa
      Observed band size: 44 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution

      Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
      Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
      Lane 5 : Mouse brain lysate
      Lane 6 : Mouse spleen lysate
      Lane 7 : Rat spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 44 kDa
      Observed band size: 44 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1-4: 30 seconds Lane 5-7: 3 minutes.

    • ALKBH1 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab195376 at 1/30 dilution.

      Western blot was performed from the immunoprecipitate using ab195376 at 1/1000 dilution.

      VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

      Lane 1: K562 whole cell lysate 10µg (Input).

      Lane 2: ab195376 IP in K562 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195376 in K562 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    References

    ab195376 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK-293T total cell lysate)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    40 µg
    Treatment
    control siRNA transfection- cells transfected with a random siRNA sequence for 48 hrs
    Specification
    HEK-293T total cell lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 02 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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