Recombinant
RabMAb

Anti-ALKBH1 antibody [EPR6176] (ab126596)

Overview

  • Product name
    Anti-ALKBH1 antibody [EPR6176]
    See all ALKBH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6176] to ALKBH1
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ALKBH1 aa 350 to the C-terminus. The exact sequence is proprietary.

  • Positive control
    • WB: Jurkat, K562 and A549 cell lysates. IHC-P: Human kidney and liver tissues. IP: Jurkat and K562 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 2.80 x 10 -11 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR6176
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localization
      Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABH antibody
      • ABH1 antibody
      • alkB antibody
      • alkB homolog 1 histone H2A dioxygenase antibody
      • AlkB, alkylation repair homolog 1 (E. coli) antibody
      • ALKB1_HUMAN antibody
      • ALKBH antibody
      • ALKBH1 antibody
      • Alkylated DNA repair protein alkB homolog 1 antibody
      • Alkylation repair homolog 1 antibody
      • Alkylation repair, alkB homolog antibody
      • Alpha-ketoglutarate-dependent dioxygenase ABH1 antibody
      • DNA lyase ABH1 antibody
      • DNA oxidative demethylase ALKBH1 antibody
      • hABH antibody
      see all

    Images

    • All lanes : Anti-ALKBH1 antibody [EPR6176] (ab126596) at 1/10000 dilution (purified)

      Lane 1 : Jurkat whole cell lysate
      Lane 2 : K562 whole cell lysate
      Lane 3 : A549 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 43 kDa
      Observed band size : 43 kDa
      Blocking and dilution buffer: 5% NFDM/TBST
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling ALKBH1 with purified ab126596 at a dilution of 1/50. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • ab126596 (purified) at a dilution of 1/30 immunoprecipitating ALKBH1 in Jurkat whole cell lysate.

      Lane 1 (input): Jurkat whole cell lysate (10µg)

      Lane 2 (+): ab126596 + Jurkat whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126596 in Jurkat whole cell lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • ab126596 (purified) at a dilution of 1/30 immunoprecipitating ALKBH1 in K562 whole cell lysate.

      Lane 1 (input): K562 whole cell lysate (10µg)

      Lane 2 (+): ab126596 + K562 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126596 in K562 whole cell lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-ALKBH1 antibody [EPR6176] (ab126596) at 1/1000 dilution (unpurified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 43 kDa
      Observed band size : 43 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ALKBH1 with unpurified ab126596 at a dilution of 1/50.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126596 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab126596.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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