Anti-ALKBH1 antibody [hABH1-151] (ab49738)

Overview

  • Product nameAnti-ALKBH1 antibody [hABH1-151]
    See all ALKBH1 primary antibodies
  • Description
    Mouse monoclonal [hABH1-151] to ALKBH1
  • SpecificityThis antibody does not recognize hABH2 or hABH3.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human ALKBH1 aa 19-34 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    GE DAFRKLFRFY RQSR

  • Positive control
    • A total cell extract of 293T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone numberhABH1-151
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 43 kDa.

Target

  • FunctionDioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localizationMitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABH antibody
    • ABH1 antibody
    • alkB antibody
    • alkB homolog 1 histone H2A dioxygenase antibody
    • AlkB, alkylation repair homolog 1 (E. coli) antibody
    • ALKB1_HUMAN antibody
    • ALKBH antibody
    • ALKBH1 antibody
    • Alkylated DNA repair protein alkB homolog 1 antibody
    • Alkylation repair homolog 1 antibody
    • Alkylation repair, alkB homolog antibody
    • Alpha-ketoglutarate-dependent dioxygenase ABH1 antibody
    • DNA lyase ABH1 antibody
    • DNA oxidative demethylase ALKBH1 antibody
    • hABH antibody
    see all

References for Anti-ALKBH1 antibody [hABH1-151] (ab49738)

ab49738 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20000 cells
Gel Running Conditions Reduced Denaturing (12.5%)
Sample Rat Tissue lysate - whole (heart, brain, muscle)
Specification heart, brain, muscle
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Dec 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"