Overview

  • Product name
  • Description
    Rabbit polyclonal to ALKBH4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 38-282 of Human ALKBH4 (BC002820).

  • Positive control
    • Human fetal spleen, fetal liver and fetal kidney lysates; Human fetal pancreas tissue.

Properties

  • Form
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/100 - 1/500.

Target

  • Function
    Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. May be involved in transcription regulation.
  • Tissue specificity
    Widely expressed, with highest expression in pancreas, ovary and spleen.
  • Sequence similarities
    Belongs to the alkB family.
  • Cellular localization
    Cytoplasm. Nucleus. Midbody. Associates with the contractile ring and midbody.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010004B12Rik antibody
    • ABH4 antibody
    • Abj4 antibody
    • AlkB, alkylation repair homolog 4 (E. coli) antibody
    • ALKB4_HUMAN antibody
    • Alkbh4 antibody
    • Alkylated DNA repair protein alkB homolog 4 antibody
    • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4 antibody
    • FLJ20013 antibody
    • Probable alpha-ketoglutarate dependent dioxygenase ABH4 antibody
    • RGD1308608 antibody
    see all

Anti-ALKBH4 antibody images

  • All lanes : Anti-ALKBH4 antibody (ab113500) at 1/500 dilution

    Lane 1 : Human fetal spleen lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human fetal kidney lysate


    Predicted band size : 34 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab113500, at 1/100 dilution, staining ALKBH4 in Formalin-fixed, Paraffin-embedded Human fetal pancreas tissue by Immunohistochemistry.
    Note cytoplasmic and nuclear staining.

References for Anti-ALKBH4 antibody (ab113500)

ab113500 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113500.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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