Overview

  • Product nameAnti-ALKBH5 antibody
    See all ALKBH5 primary antibodies
  • Description
    Mouse polyclonal to ALKBH5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human OFOXD protein (NP_060228).

  • Positive control
    • OFOXD transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab69325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • RelevanceDioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export and is required for spermatogenesis.
  • Cellular localizationNucleus speckle
  • Database links
  • Alternative names
    • ABH5 antibody
    • AlkB, alkylation repair homolog 5 (E. coli) antibody
    • AlkB, alkylation repair homolog 5 antibody
    • Alkylated DNA repair protein alkB homolog 5 antibody
    • Alpha ketoglutarate dependent dioxygenase alkB homolog 5 antibody
    • OFOXD antibody
    • OFOXD1 antibody
    • Oxoglutarate and iron-dependent oxygenase domain containing antibody
    • Probable alpha ketoglutarate dependent dioxygenase ABH5 antibody
    • RNA demethylase ALKBH5 antibody
    see all

Anti-ALKBH5 antibody images

  • All lanes : Anti-ALKBH5 antibody (ab69325) at 1/500 dilution

    Lane 1 : ALKBH5 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa

References for Anti-ALKBH5 antibody (ab69325)

This product has been referenced in:
  • Karkhanis V  et al. Protein Arginine Methyltransferase 7 Regulates Cellular Response to DNA Damage by Methylating Promoter Histones H2A and H4 of the Polymerase d Catalytic Subunit Gene, POLD1. J Biol Chem 287:29801-14 (2012). WB ; Human . Read more (PubMed: 22761421) »

See 1 Publication for this product

Product Wall

Thank you for your reply. I am happy to hear that the positive control has helped with your other antibodies. Yes, K-562 cell lysate will be a suitable positive control for ALKBH5 as well. I hope this helps, please let me know if you have any other que...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"