The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionProbable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron.
Tissue specificityWidely expressed, with highest expression in pancreas, followed by spleen, prostate, ovary and placenta.
Sequence similaritiesBelongs to the alkB family.
Cellular localizationCytoplasm. Nucleus. Secreted. Has a predicted N-terminal signal sequence, indicating it may be secreted. Detected in cytoplasm and nucleus when expressed as fusion protein with an N-terminal tag.