Overview

  • Product name
  • Description
    Rabbit polyclonal to ALKBH7
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GQTLLSSVHV LDLEARGYIK PHVDSIKFCG ATIAGLSLLS PSVMRLVHTQ EPGEWLELLL EPGSLYILRG SARYDFSHEI LRDEESFFG, corresponding to amino acids 100-188 of Human ALKBH7.

  • Positive control
    • Human pancreas tissue; ALKBH7 over-expression HEK293T Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab122596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron.
  • Tissue specificity
    Widely expressed, with highest expression in pancreas, followed by spleen, prostate, ovary and placenta.
  • Sequence similarities
    Belongs to the alkB family.
  • Cellular localization
    Cytoplasm. Nucleus. Secreted. Has a predicted N-terminal signal sequence, indicating it may be secreted. Detected in cytoplasm and nucleus when expressed as fusion protein with an N-terminal tag.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABH7 antibody
    • ALKB7_HUMAN antibody
    • ALKBH7 antibody
    • Alkylated DNA repair protein alkB homolog 7 antibody
    • MGC10974 antibody
    • Probable alpha-ketoglutarate-dependent dioxygenase ABH7 antibody
    • SPATA11 antibody
    • Spermatogenesis cell proliferation-related protein antibody
    • Spermatogenesis-associated protein 11 antibody
    • UNQ6002 antibody
    • UNQ6002/PRO34564 antibody
    see all

Images

  • ab122596, at 1/20 dilution, staining ALKBH7 in paraffin embedded Human pancreas by Immunohistochemistry.

References

ab122596 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - Triton
Fixative
Formaldehyde
Username

Herr Dr. Alexander Wolf

Verified customer

Submitted Jul 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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